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Soderstrom E McKenna JA Abrams GS Adolphsen CE Averill D Ballam J Barish BC Barklow T Barnett BA Bartelt J Bethke S Blockus D Bonvicini G Boyarski A Brabson B Breakstone A Bulos F Burchat PR Burke DL Cence RJ Chapman J Chmeissani M Cords D Coupal DP Dauncey P DeStaebler HC Dorfan DE Dorfan JM Drewer DC Elia R Feldman GJ Fernandes D Field RC Ford WT Fordham C Frey R Fujino D Gan KK Gero E Gidal G Glanzman T Goldhaber G Gomez Cadenas JJ Gratta G Grindhammer G Grosse-Wiesmann P Hanson G Harr R 《Physical review letters》1990,64(25):2980-2983
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Komamiya S Abrams GS Adolphsen CE Averill D Ballam J Barish BC Barklow T Barnett BA Bartelt J Bethke S Blockus D Bonvicini G Boyarski A Brabson B Breakstone A Bulos F Burchat PR Burke DL Cence RJ Chapman J Chmeissani M Cords D Coupal DP Dauncey P DeStaebler HC Dorfan DE Dorfan JM Drewer DC Elia R Feldman GJ Fernandes D Field RC Ford WT Fordham C Frey R Fujino D Gan KK Gatto C Gero E Gidal G Glanzman T Goldhaber G Gomez Cadenas JJ Gratta G Grindhammer G Grosse-Wiesmann P Hanson G Harr R 《Physical review letters》1990,64(24):2881-2884
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Blaylock GT Bolton T Brown JS Bunnell KO Burnett TH Cassell RE Coffman D Cook V Coward DH Dorfan DE Dubois GP Eigen G Eisenstein BI Freese T Gladding G Grab C Heusch CA Hitlin DG Izen JM Köpke L Li A Lockman WS Mallik U Matthews CG Mir R Mockett PM Mozley RF Nemati B Odian A Parker J Parrish L Partridge R Pitman D Sadrozinski HF Scarlatella M Schalk TL Schindler RH Seiden A Simopoulos C Stockdale IE Stockhausen W Thaler JJ Toki W Tripsas B Villa F Wasserbaech S Wattenberg A Weinstein AJ 《Physical review letters》1987,58(21):2171-2174
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Thaler F Valsasina B Baldi R Xie J Stewart A Isacchi A Kalisz HM Rusconi L 《Analytical and bioanalytical chemistry》2003,376(3):366-373
beta-Elimination of the phosphate group on phosphoserine and phosphothreonine residues and addition of an alkyldithiol is a useful tool for analysis of the phosphorylation states of proteins and peptides. We have explored the influence of several conditions on the efficiency of this PO(4)(3-) elimination reaction upon addition of propanedithiol. In addition to the described influence of different bases, the solvent composition was also found to have a major effect on the yield of the reaction. In particular, an increase in the percentage of DMSO enhances the conversion rate, whereas a higher amount of protic polar solvents, such as water or isopropanol, induces the opposite effect. We have also developed a protocol for enrichment of the modified peptides, which is based on solid-phase covalent capture/release with a dithiopyridino-resin. The procedure for beta-elimination and isolation of phosphorylated peptides by solid-phase capture/release was developed with commercially available alpha-casein. Enriched peptide fragments were characterized by MALDI-TOF mass spectrometric analysis before and after alkylation with iodoacetamide, which allowed rapid confirmation of the purposely introduced thiol moiety. Sensitivity studies, carried out in order to determine the detection limit, demonstrated that samples could be detected even in the low picomolar range by mass spectrometry. The developed solid-phase enrichment procedure based on reversible covalent binding of the modified peptides is more effective and significantly simpler than methods based on the interaction between biotin and avidin, which require additional steps such as tagging the modified peptides and work-up of the samples prior to the affinity capture step. 相似文献
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Cinabro D Henderson S Liu T Saulnier M Wilson R Yamamoto H Bergfeld T Eisenstein BI Gollin G Ong B Palmer M Selen M Thaler JJ Sadoff AJ Ammar R Ball S Baringer P Bean A Besson D Coppage D Copty N Davis R Hancock N Kelly M Kwak N Lam H Kubota Y Lattery M Nelson JK Patton S Perticone D Poling R Savinov V Schrenk S Wang R Alam MS Kim IJ Nemati B O'Neill JJ Severini H Sun CR Zoeller MM Crawford G Daubenmier CM Fulton R Fujino D Gan KK Honscheid K Kagan H Kass R Lee J Malchow R Morrow F Skovpen Y 《Physical review letters》1994,72(10):1406-1410