Dynamic specification of coarticulated vowels spoken in sentence context

According to a dynamic specification account, coarticulated vowels are identified on the basis of time-varying acoustic information, rather than solely on the basis of "target" information contained within a single spectral cross section of an acoustic syllable. Three experiments utilizing digitally segmented portions of consonant-vowel-consonant (CVC) syllables spoken rapidly in a carrier sentence were designed to examine the relative contribution of (1) target information available in vocalic nuclei, (2) intrinsic duration information specified by syllable length, and (3) dynamic spectral information defined over syllable onsets and offsets. In experiments 1 and 2, vowels produced in three consonantal contexts by an adult male were examined. Results showed that vowels in silent-center (SC) syllables (in which vocalic nuclei were attentuated to silence leaving initial and final transitional portions in their original temporal relationship) were perceived relatively accurately, although not as well as unmodified syllables (experiment 1); random versus blocked presentation of consonantal contexts did not affect performance. Error rates were slightly greater for vowels in SC syllables in which intrinsic duration differences were neutralized by equating the duration of silent intervals between initial and final transitional portions. However, performance was significantly better than when only initial transitions or final transitions were presented alone (experiment 2). Experiment 3 employed CVC stimuli produced by another adult male, and included six consonantal contexts. Both SC syllables and excised syllable nuclei with appropriate intrinsic durations were identified no less accurately than unmodified controls. Neutralizing duration differences in SC syllables increased identification errors only slightly, while truncating excised syllable nuclei yielded a greater increase in errors. These results demonstrate that time-varying information is necessary for accurate identification of coarticulated vowels. Two hypotheses about the nature of the dynamic information specified over syllable onsets and offsets are discussed.     

Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase

Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone.     

Ultraviolet radiation, vitamin D and risk of prostate cancer and other diseases

Most common diseases appear to result from complex, poorly understood interactions between genetic and environmental factors. Relatively few factors have been unequivocally linked with disease risk or outcome. Evidence from various studies using different experimental approaches has been interpreted as showing that, apart from its harmful effects on the pathogenesis of the common skin cancers, ultraviolet radiation (UVR) may exert a beneficial effect on development of various internal cancers and other pathologies. This concept is supported by parallel studies showing that hypovitaminosis D is linked with increased risk of various diseases including insulin resistance and multiple sclerosis. These findings suggest that, first, host factors such as skin pigmentation that affect UVR-induced synthesis of vitamin D and, second, polymorphism in genes that mediate the effectiveness of vitamin D action are susceptibility candidates for a variety of diseases. Collectively, these data suggest the hypothesis that, via effects on vitamin D synthesis, UVR exposure has beneficial effects on susceptibility and outcome to a variety of complex diseases. We describe evidence from studies in various diseases, but mainly from prostate cancer patients, that supports this hypothesis, but we emphasize that, although supportive data are available, the concept is unproven. Indeed, other explanations are possible. However, given the potentially important public health implications of the hypothesis and the potential for the development of novel therapeutic modalities, we believe the concept is worthy of further investigation.     

Separation of chlamydosporol epimers by reversed-phase HPLC using commercial solvent optimisation software

Summary Two epimers of the mycotoxin chlamydosporol were separated by HPLC on an RP-18 column using a quaternary mobile phase consisting of water (79.1%), methanol (10.0%), acetonitrile (10.4%) and tetrahydrofuran (0.5%), with a flow rate of 1 ml min^–1. This optimal composition of mobile phase, with which the resolution value for the two epimers (1 and2) was 2.73 with retention times of 5.88 and 7.12 min, respectively, was achieved by the application of Philips Solvent Optimisation Software PU 6100. The presence of free silanols on the stationary phase was shown to be an essential requirement for the separation of the chlamydosporol epimers.     

Immobilizing a Fluorescent Dye Offers Potential to Investigate the Glass/Resin Interface

Silane coupling agents are commonly applied to glass fibers to promote fiber/resin adhesion and enhance durability in composite parts. In this study, a coupling agent multilayer on glass was doped with trace levels of the dimethylaminonitrostilbene (DMANS) fluorophore. The fluorophore was immobilized on the glass surface by tethering the molecule to a triethoxy silane coupling agent, creating the DMANS/silane coupling agent molecule (DMSCA). DMSCA was then diluted with commonly used coupling agents and grafted to a glass microscope coverslip to create a model composite interface. A 53-nm blue shift in fluorescence from the immobilized DMSCA can be followed during cure of an epoxy resin overlayer, giving this technique potential to monitor the properties of the fiber/resin interface during composite processing. Contact angle measurements on these coupling agent layers were similar in the presence or absence of the DMSCA molecule, suggesting that trace levels of the fluorescent probe did not affect the structure of the layer. The immobilized DMSCA molecule behaved similarly to the DMANS precursor in solution. Both showed longer wavelength fluorescence in more polar environments. Copyright 2000 Academic Press.