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1.
KL Britton HF Rogers Y Asano T Dairi Y Kato TJ Stillman DW Rice 《Acta crystallographica. Section D, Biological crystallography》1998,53(4):124-126
The novel NAD+-linked opine dehydrogenase from a soil isolate Arthrobacter sp. strain 1C belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. Crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 ? resolution. X-ray precession photographs have established that the crystals belong to space group P21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 ? and a single subunit in the asymmetric unit. The elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this novel class of dehydrogenases to enable a comparison to be made with other enzyme families and also as the basis for mutagenesis experiments directed towards the production of natural and synthetic opine-type compounds containing two chiral centres. 相似文献
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Anomalous fading of TL, OSL and IRSL has been observed in many samples of feldspars and attributed to the tunnel effect. Investigations do show expected tunnel afterglow except for samples with no fading. Its intensitym quite noticeable at LNT, is in proportion with reported rate of fading. The emission is entirely in the red and infrared part of the spectrum. An important thermal quenching is observed. Low temperature storage results in fading of TL. Cathodoluminescence emission spectra, monitored from 200 to 900 nm, do confirm TL observations. They show two well-separated ranges: one, “blue”, from UV to yellow (the only one observed in usual TL dating), displays various emission bands; the other, “IR”, red and infrared, shows a well-characterized narrow Gaussian emission band, with a maximum around 720 nm. Models are proposed, relating fading with disorder in crystals. Tunnel afterglow appears as a good criterion of fading in feldspars. 相似文献
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Cracks that propagate with near-perfect sinusoidal form are reported in amorphous silicon-rich silica films deposited onto (001) silicon substrates by plasma-enhanced chemical vapour deposition and subjected to thermal annealing. The cracks are shown to result from high tensile stresses that develop in the film during thermal annealing at temperatures in the range up to 700°C, a process shown to be correlated with the loss of hydrogen from the films. Two distinct modes of crack propagation are reported: straight cracks that propagate along directions parallel to [100] cube-edge directions in the substrate, and oscillating cracks that propagate with sinusoidal form parallel to [110] diagonal directions. Sections through the cracks show that the oscillating cracks have a complex three-dimensional structure that extends through the glassy film and into the underlying silicon substrate. This involves a correlated oscillation between the crystallographic orientation of the crack in the surface plane and that of the crack extension into the substrate. Whereas a complete theoretical treatment of this behaviour would be extremely complicated, a simple theory is developed to demonstrate that an oscillating crack has a minimum energy per unit length for a particular wavelength and amplitude that depends upon the physical parameters of both film and substrate. The energy at this minimum is shown to be lower than that of a straight crack for certain parameter ranges so that the oscillating geometry is preferred. 相似文献
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M. Brouillette M. Doré C. Hébert M.-F. Spooner S. Marchand J. Côté F. Gobeil M. Rivest M. Lafrance B. G. Talbot J.-M. Moutquin 《Shock Waves》2016,26(1):25-37
We present a novel intradermal needle-free drug delivery device which exploits the unsteady high-speed flow produced by a miniature shock tube to entrain drug or vaccine particles onto a skin target. A first clinical study of pain and physiological response of human subjects study is presented, comparing the new injector to intramuscular needle injection. This clinical study, performed according to established pain assessment protocols, demonstrated that every single subject felt noticeably less pain with the needle-free injector than with the needle injection. Regarding local tolerance and skin reaction, bleeding was observed on all volunteers after needle injection, but on none of the subjects following powder injection. An assessment of the pharmacodynamics, via blood pressure, of pure captopril powder using the new device on spontaneously hypertensive rats was also performed. It was found that every animal tested with the needle-free injector exhibited the expected pharmacodynamic response following captopril injection. Finally, the new injector was used to study the delivery of an inactivated influenza vaccine in mice. The needle-free device induced serum antibody response to the influenza vaccine that was comparable to that of subcutaneous needle injection, but without requiring the use of an adjuvant. Although no effort was made to optimize the formulation or the injection parameters in the present study, the novel injector demonstrates great promise for the rapid, safe and painless intradermal delivery of systemic drugs and vaccines. 相似文献
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de Prinse Thomas Klantsataya Elizaveta Tsiminis Georgios Payten Thomas Moffatt Jillian Kee Tak W. Spooner Nigel A. 《Journal of fluorescence》2022,32(3):1051-1057
Journal of Fluorescence - Acetone and butanone were seen to emit blue light around 450 nm when excited in the green by a high intensity pulsed laser. The pathway of this anti-Stokes... 相似文献
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