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L Soltés 《Biomedical chromatography : BMC》1992,6(1):43-49
The paper presents results and experience obtained in the isolation of a number of basic polar/non-polar solutes (drugs, their metabolites and structural derivatives) from biological samples by using the separation minicolumns Separcol SI and Separcol SI C18 L/T. The aim was to provide insight into some simple procedures yielding optimal selectivity/specificity of the isolation of the studied solutes either by proper selection of the sorbent or by applying sophisticated elution schemes which use to advantage different properties of the eluents/solvents. 相似文献
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4.
G. Solt 《Hyperfine Interactions》1995,96(1):167-175
The Kubo-Toyabe semiclassical formula, describing the time development of the polarization of a particle in zero external field at a lattice site with cubic local environment, is generalized for uniaxial site symmetry. The relaxation function and, in particular, its first moments and long time asymptotics obtained in a closed form depend on the angle between polarization and the crystalc-axis and are shown to vary sensitively with the asymmetry of the field distribution at the particular muon site. Besides the exact uniaxial variant of the Kubo-Toyabe relaxation function, an approximate simple interpolation formula is also derived, which is correct for both short times and in its long time asymptotics. The two parameters (, 1) in the uniaxial formulae can be determined by using the observed values of the second momentM
2 for two different crystal orientations. 相似文献
5.
Rafael Abela Christopher Baines Xavier Donath Dierk Herlach David Maden Ivan D. Reid Dieter Renker György Solt Ulrich Zimmermann 《Hyperfine Interactions》1994,87(1):1105-1110
TheSR Facility Instruments presently available at PSI and the envisaged medium- and long-term developments are presented. The plans focus on further upgrades of the existing instruments and the development of new techniques using the very high fluxes becoming available at PSI, in particular the setup of a beamline with a fast kicker for muons on request (MORE) and the development of very low energy muon beams. 相似文献
6.
Kyselová Z Kysel'ová Z Krizanová L Soltés L Stefek M 《Journal of chromatography. A》2005,1084(1-2):95-100
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of eye lens proteins showed that both progression of diabetic cataract in rats in vivo and precipitation of soluble eye lens proteins stressed by free radicals in vitro were accompanied by significant protein cross-linking. There was a noticeable contribution of disulfide bridges to protein cross-linking in diabetic eye lens in vivo. In contrast, under conditions in vitro, when eye lens proteins were exposed to hydroxyl or peroxyl radicals, we showed that the participation of reducible disulfide linkages in the formation of high molecular mass products was markedly lower. These in vivo--in vitro differences indicate that the generally accepted role of reactive oxygen species in diabetic cataractogenesis may be overestimated in connection with the processes of protein cross-linking. 相似文献
7.
This overview provides comprehensive information on the most relevant results of Stobadine preclinical disposition studies. In order to investigate pharmacokinetic processes of the drug in rats, dogs and in human volunteers, several bioanalytical assays based on radiometric, spectrofluorometric, as well as chromatographic determination methods were developed and implemented. In small laboratory animals, the drug absorption, distribution, metabolism and elimination were investigated by administering 3H-labeled Stobadine. Spectrofluorometry was used alternatively for the determination of cold/unlabeled Stobadine in extracts of biomaterials sampled from larger animal species. The chromatographic separation methods proved, however, to be the most advantageous for determining details of the drug disposition and fate in the body. 相似文献
8.
Sonication of chitin-glucan, preparation of water-soluble fractions and characterization by HPLC 总被引:1,自引:0,他引:1
A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography. 相似文献
9.
Ohne ZusammenfassungXV. Mitteilung: M. Kohn und A. Rosenfeld, Ein Beitrag zur Kenntnis der Pseudophenole. Monatshefte f. Chemie, 1925. 相似文献
10.
A strongly perturbing defect in a metal, like a H+ particle, is not only a force centre repelling or attracting its neighbours but it modifies considerably the interatomic forces them. The relevant quantity determining the nature of these forces is, in metals, the dielectric susceptibility of the inhomogeneous impurity-in-jellium system. The absence of translational symmetry in the polarizability leads to a ‘bipolar’ screening and, thereby, to non-central interactions between the neighbouring atoms. Exact asymptotic formulae have been found for the independent-particle polarizability including bound and scattered states, assuming predominantly s-scattering at the impurity. A semi-quantitative study of the atomic interactions in the ‘impurity-in-jellium’ background is given for a simplified theoretical model which allows for an analytic approach and is, nevertheless, expected to reproduce the main features of forces in a dilute hydride system. 相似文献