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1.
V. A. Fedorchuk E. S. Puchkovskaya L. S. Anisimova G. B. Slepchenko 《Journal of Analytical Chemistry》2005,60(6):518-522
Conditions were selected for the voltammetric determination of streptomycin and azitromycin using electrodes of different types. The interfering effects of cations, anions, and organic substances, food proteins among them, on the analytical signals from antibiotics were estimated. Procedures for determining streptomycin and azitromycin in pharmaceuticals and for determining streptomycin in milk at nano levels were developed.__________Translated from Zhurnal Analiticheskoi Khimii, Vol. 60, No. 6, 2005, pp. 586–591.Original Russian Text Copyright © 2005 by Fedorchuk, Puchkovskaya, Anisimova, Slepchenko.Presented at the VI All-Russian Conference (with international participation) on Electrochemical Methods of Analysis (EMA-2004, Ufa, May 23–27, 2004). 相似文献
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G. B. Slepchenko T. M. Gindullina S. V. Nekhoroshev 《Journal of Analytical Chemistry》2017,72(7):703-709
A survey of the capabilities of electrochemical methods for the separation (capillary electrophoresis) and determination (amperometry, the versions of voltammetry, and potentiometry) of narcotic and psychotropic drugs and their metabolites, which are of interest in criminological practice, as the constituents of biological materials (hair, blood, and urine) and medicinal preparations is presented. 相似文献
3.
Olga Mezentseva Galina Slepchenko Victor Filimonov Elena Mikheeva Galina Arbit 《Electroanalysis》2019,31(8):1477-1483
The electrochemical behavior of a number of benzoyl barbiturates was studied using a glassy carbon electrode (GCE). The kinetics of the electrode process is determined, the contribution of physical adsorption to the electrochemical process is estimated, and the mechanism of the possible electrochemical reaction is proposed. It is shown that the electrochemical reduction potentials of benzoyl phenobarbital derivatives are determined by the LUMO energies, calculated by the B3LYP 6‐311+G method. It is established that the process is quasi‐reversible, complicated by adverse reactions. The influence of halogen type and its position in the benzoyl residue of the studied substances on the analytical signal is established. The effective values of the dissociation constants of various forms of benzoyl derivatives were calculated using the example of halonal, for which the values 3.16 ? 10?8 and 6.31 ? 10?12, respectively, were found. 相似文献
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Olga L. Mezentseva Galina B. Slepchenko Elena V. Dorozhko Eugenia S. Moiseeva Vladimir P. Kryukovskiy Nikolay K. Zaycev 《Electroanalysis》2023,35(4):e202200252
A highly sensitive electrochemical sensor based on arenediazonium tosylates was designed to detect meldonium in urine. The effect of the concentration of ArN2+OTs− arenediazonium tosylate modifier and various substituents was studied. Basic operating parameters for voltammetric meldonium detection were established and the procedure for urine sample preparation was developed. The following values were obtained: limit of detection (LOD) 0.005 mg ⋅ L−1 (P=0.95) and limit of quantification (LOQ) 0.01 mg ⋅ L−1. The relationship between the analytical signal and meldonium concentration in the solution ranging from 0.01 to 400 mg ⋅ L−1 was described by a linear function. The meldonium concentration error did not exceed 18 %. The analysis time for a single urine sample was reduced to 15 minutes. 相似文献
6.
Elena V. Dorozhko Nikita A. Lunev Elena I. Korotkova Galina B. Slepchenko Olga I. Lipskikh Ekaterina I. Mikhnevich 《Electroanalysis》2023,35(9):e202300054
In this work two strategies for the synthesis of peroxidase silver conjugates for the qualitative and quantitative determination of immunoglobulins (IgG) to ixodid tick-borne borreliosis (ITBB) (Lyme disease) in human serum were proposed. The first approach for Ab-HRP@AgNP conjugate synthesis involved silver nanoparticles (Ag NPs) capped with a commercial peroxidase conjugate (Ab-HRP) by passive adsorption. The second strategy was based on the initial coupling of Ag NPs with human anti-species antibodies (Ab) by passive adsorption followed by the introduction of horseradish peroxidase (HRP) enzyme into the reaction mixture as a blocking reagent for Ab@AgNP@HRP conjugate synthesis. The formation of peroxidase silver conjugates was proved by UV/Vis spectroscopy and Transmission Electron Microscopy (TEM). The catalytic activity of Ab-HRP@AgNP and Ab@AgNP@HRP conjugates was evaluated by Michaelis-Menten kinetics. A commercially available 96-well microtiter plate with recombinant antigens to ITBB was used as a platform for immobilization of analyzed IgG. The HRP in Ab-HRP@AgNP conjugate was found to retain a sufficient level of activity for interaction with the H2O2 substrate to form an intensely colored reaction product. Therefore Ab-HRP@AgNP conjugate can be used in enzyme-linked immunosorbent assay (ELISA) with spectrophotometric detection of 3,3’,5,5’-Tetramethylbenzidine (TMB Ox) for quantitative determination of IgG to ITBB in human serum in the concentration range 12.5–800 ng ml−1 with LOD 2 ng ml−1. Ab@AgNP@HRP conjugate is recommended for the electrochemical determination of IgG to ITBB in human serum at LOD 3 ng ml−1 with registration of silver oxidation by linear sweep anodic stripping voltammetry (LSASV). Ag NPs in Ab-HRP@AgNP and Ab@AgNP@HRP conjugates do not change electrochemical activity during storage and can be used as an electrochemical label in LSASV method in case of HRP inactivation. The immunoassay based on peroxidase silver conjugates expands the analytical potential for the determination of IgG to ITBB especially during the period of increasing incidence. 相似文献
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Electrodiffusion of ions, both inside and outside biologicalcells, are of utmost importance to proper cellular functions.Experiments indicate that both ion concentrations and electropotentialcan jump discontinuously across the cell membranes. We studya system of nonlinear partial differential equations modellingsuch phenomena. Jump conditions for species concentrations andelectropotential across cell membranes are imposed. Under zero-fluxboundary conditions for one-dimensional domains, the solutionsare proved to exist for all times. With further assumptions,these transient solutions will converge to the unique steady-statesolution. Numerical experiments in one- and two-dimensionaldomains are also performed in order to study some unresolvedtheoretical issues. 相似文献
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E. V. Mikheeva O. A. Martynyuk G. B. Slepchenko L. S. Anisimova 《Journal of Analytical Chemistry》2009,64(7):731-734
The electrochemical behavior of vitamin B2 at a glassy-carbon electrode is studied. Some physicochemical parameters of the electrode reaction (pK values for different forms of vitamin B2 and the number of electrons involved in the electrode process) are calculated. A rapid procedure is developed for the quantitative chemical determination of vitamin B2 in breast milk by differential voltammetry. 相似文献
9.
O. G. Filichkina E. A. Zakharova G. B. Slepchenko 《Journal of Analytical Chemistry》2004,59(5):481-486
A universal procedure was developed for the voltammetric determination of total selenium in various foodstuffs. The procedure involves the mineralization of a test sample with magnesium nitrate followed by the reduction of selenium(VI) to selenium(IV) in 6 M HCl and the determination of selenium by cathodic square-wave voltammetry at a mercury–graphite electrode in an HCl solution in the presence of copper and mercury ions. 相似文献
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