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Johannsen MW Crispino L Wamberg MC Kalra N Wengel J 《Organic & biomolecular chemistry》2011,9(1):243-252
The synthesis of 2'-amino-LNA (the 2'-amino derivative of locked nucleic acid) has opened up a number of exciting possibilities with respect to modified nucleic acids. While maintaining the excellent duplex stability inferred by LNA-type oligonucleotides, the nitrogen in the 2'-position of 2'-amino-LNA monomers provides an excellent handle for functionalisation. Herein, the synthesis of amino acid functionalised 2'-amino-LNA derivatives is described. Following ON synthesis, a glycyl unit attached to the N2'-position of 2'-amino-LNA monomers was further acylated with a variety of amino acids. On binding to DNA/RNA complements, the modified ONs induce a marked increase in thermal stability, which is particularly apparent in a buffer system with a low salt concentration. The increase in thermal stability is thought to be caused, at least in part, by decreased electrostatic repulsion between the negatively charged phosphate backbones when positively charged amino acid residues are appended. Upon incorporation of more than one 2'-amino-LNA modification, the effects are found to be nearly additive. For comparison, 2'-amino-LNA derivatives modified with uncharged groups have been synthesised and their effect on duplex thermal stability likewise investigated. 相似文献
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Cera G Crispino P Monari M Bandini M 《Chemical communications (Cambridge, England)》2011,47(27):7803-7805
A reliable synthetic route to fused polycyclic indolines is documented by the development of a stereoselective gold catalyzed cascade cyclization of indole propargylic alcohols. 相似文献
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Cletus A D'Souza Vikramjit Chopra Richard Varhol Yuan-Yun Xie Slavita Bohacec Yongjun Zhao Lisa LC Lee Mikhail Bilenky Elodie Portales-Casamar An He Wyeth W Wasserman Daniel Goldowitz Marco A Marra Robert A Holt Elizabeth M Simpson Steven JM Jones 《BMC neuroscience》2008,9(1):1-14
Background
We have recorded responses from single neurons in murine visual cortex to determine the effectiveness of the input from the two murine cone photoreceptor mechanisms and whether there is any unique selectivity for cone inputs at this higher region of the visual system that would support the possibility of colour vision in mice. Each eye was stimulated by diffuse light, either 370 (strong stimulus for the ultra-violet (UV) cone opsin) or 505 nm (exclusively stimulating the middle wavelength sensitive (M) cone opsin), obtained from light emitting diodes (LEDs) in the presence of a strong adapting light that suppressed the responses of rods.Results
Single cells responded to these diffuse stimuli in all areas of striate cortex. Two types of responsive cells were encountered. One type (135/323 – 42%) had little to no spontaneous activity and responded at either the on and/or the off phase of the light stimulus with a few impulses often of relatively large amplitude. A second type (166/323 – 51%) had spontaneous activity and responded tonically to light stimuli with impulses often of small amplitude. Most of the cells responded similarly to both spectral stimuli. A few (18/323 – 6%) responded strongly or exclusively to one or the other spectral stimulus and rarely in a spectrally opponent manner.Conclusion
Most cells in murine striate cortex receive excitatory inputs from both UV- and M-cones. A small fraction shows either strong selectivity for one or the other cone mechanism and occasionally cone opponent responses. Cells that could underlie chromatic contrast detection are present but extremely rare in murine striate cortex. 相似文献6.
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Epitaxial La1−x
Pb
x
MnO3 (LPMO) thin films, grown on (100) SrTiO3 substrates by laser ablation technique at different temperatures between 600 and 850°C, have been characterized for electrical
and magnetic properties. The temperature dependence of resistivity showed that the metal-insulator transition temperature
(T
MI) decreases with increasing substrate temperature, which has been attributed to decrease in Pb content in the filsm. The YBa2Cu3O
x
/La1−x
MnO3 heterostructures, exhibiting both superconductivity and ferromagnetism, have been fabricated. 相似文献
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In 1919, A. C. D. Crommelin and C. R. Davidson, British astronomers from the Greenwich Observatory in England, passed by Amazonia on their Brazilian journey aiming to measure the bending of stars' light rays during the total solar eclipse of May 29, 1919, and thereby put the theory of general relativity to the test. In the context of Crommelin’s and Davidson’s visit, we discuss how Amazonia was introduced to Einstein’s theory of gravitation, and also the observations and repercussions of the May 29, 1919, solar eclipse in Belém, capital city of the North-Brazilian Pará state. 相似文献