排序方式: 共有30条查询结果,搜索用时 26 毫秒
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Subdiffraction resolution in total internal reflection fluorescence microscopy with a grating substrate 总被引:1,自引:0,他引:1
We propose a fluorescence surface imaging system that presents a power of resolution beyond that of the diffraction limit without resorting to saturation effects or probe scanning. This is achieved by depositing the sample on an optimized periodically nanostructured substrate in a standard total internal reflection fluorescence microscope. The grating generates a high-spatial-frequency light grid that can be moved throughout the sample by changing the incident angle. An appropriate reconstruction procedure permits one to recover the fluorescence amplitude from the images obtained for various incidences. Simulations of this imaging system show that the resolution is not limited by diffraction but by the period of the grating. 相似文献
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Patrick C. Chaumet Anne Sentenac Anne-Laure Fehrembach 《Optical and Quantum Electronics》2017,49(2):71
In this paper, we study the performances of the Dipole Discrete Approximation method for modelling the reflectivity of a highly resonant, 3D subwavelength patterned structure extending over tens of thousands wavelength square. The computation time of the whole reflectivity spectrum (80 wavelengths) was about 3 h on a computer and requires about 1.5 GB in memory. These performances make the DDA an unique numerical tool for modelling the scattering by large 3D structures supporting long-range interactions. 相似文献
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Catarina IV Ramos Flávio Figueira Marcelo D Polêto Francisco ML Amado Hugo Verli João PC Tomé M Graça PMS Neves 《Journal of mass spectrometry : JMS》2016,51(5):342-349
Electrospray mass spectrometry/mass spectrometry was used to investigate the gas‐phase properties of protonated expanded porphyrins, in order to correlate those with their structure and conformation. We have selected five expanded meso‐pentafluorophenyl porphyrins, respectively, a pair of oxidized/reduced fused pentaphyrins (22 and 24 π electrons), a pair of oxidized/reduced regular hexaphyrins (26 and 28 π electrons) and a regular doubly N‐fused hexaphyrin (28 π electrons). The gas‐phase behavior of the protonated species of oxidized and reduced expanded porphyrins is different. The oxidized species (aromatic Hückel systems) fragment more extensively, mainly by the loss of two HF molecules. The reduced species (Möbius aromatic or Möbius‐like aromatic systems) fragment less than their oxidized counterparts because of their increased flexibility. The protonated regular doubly fused hexaphyrin (non‐aromatic Hückel system) shows the least fragmentation even at higher collision energies. In general, cyclization through losses of HF molecules decreases from the aromatic Hückel systems to Möbius aromatic or Möbius‐like aromatic systems to non‐aromatic Hückel systems and is related to an increase in conformational distortion. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
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Tomographic diffractive microscopy with a wavefront sensor 总被引:1,自引:0,他引:1
Ruan Y Bon P Mudry E Maire G Chaumet PC Giovannini H Belkebir K Talneau A Wattellier B Monneret S Sentenac A 《Optics letters》2012,37(10):1631-1633
Tomographic diffractive microscopy is a recent imaging technique that reconstructs quantitatively the three-dimensional permittivity map of a sample with a resolution better than that of conventional wide-field microscopy. Its main drawbacks lie in the complexity of the setup and in the slowness of the image recording as both the amplitude and the phase of the field scattered by the sample need to be measured for hundreds of successive illumination angles. In this Letter, we show that, using a wavefront sensor, tomographic diffractive microscopy can be implemented easily on a conventional microscope. Moreover, the number of illuminations can be dramatically decreased if a constrained reconstruction algorithm is used to recover the sample map of permittivity. 相似文献
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