A Biosilification Fusion Protein for a ‘Self‐immobilising’ Sarcosine Oxidase Amperometric Enzyme Biosensor

Monomeric sarcosine oxidase (mSOx) fusion with the silaffin peptide, R5, designed previously for easy protein production in low resource areas, was used in a biosilification process to form an enzyme layer electrode biosensor. mSOx is a low activity enzyme (10–20 U/mg) requiring high amounts of enzyme to obtain an amperometric biosensor signal, in the clinically useful range <1 mM sarcosine, especially since the K_m is >10 mM. An amperometric biosensor model was fitted to experimental data to investigate dynamic range. mSOx constructs were designed with 6H (6×histidine) and R5 (silaffin) peptide tags and compared with native mSOx. Glutaraldehyde (GA) cross‐linked proteins retained ～5 % activity for mSOx and mSOx‐6H and only 0.5 % for mSOx‐R5. In contrast R5 catalysed biosilification on (3‐mercaptopropyl) trimethoxysilane (MPTMS) and tetramethyl orthosilicate (TMOS) particles created a ‘self‐immobilisation’ matrix retaining 40 % and 76 % activity respectively. The TMOS matrix produced a thick layer (>500 μm) on a glassy carbon electrode with a mediated current due to sarcosine in the clinical range for sarcosinemia (0–1 mM). The mSOx‐R5 fusion protein was also used to catalyse biosilification in the presence of creatinase and creatininase, entrapping all three enzymes. A mediated GC enzyme linked current was obtained with dynamic range available for creatinine determination of 0.1–2 mM for an enzyme layer ～800 nm.     

非线性光纤方向耦合器孤子动力特性分析

利用变分方法,导出了双曲正割型光孤子在非线性光纤耦合器中传输时满足的动力学方程组,分析了该方程组的平衡点性态,讨论了光孤子的开关特性.指出参数|β|<2π.是能实现完全的开关操作的必要条件.     

基于Terminal滑模的有限时间混沌同步实现

提出了混沌同步有限时间实现问题，将Terminal滑模控制技术与混沌同步问题相结合，把同步问题视为响应系统的跟踪问题，给出了由线性滑模函数引入非线性项构造Terminal滑模面的充分条件，以及相应Terminal滑模控制器.以主从Duffing系统为例研究验证同步策略的可行性和有效性. 关键词： Terminal滑模 混沌同步 Duffing系统     

Soliton interaction under the influence of higher-order effects

In this paper, we present exact N-soliton solution by employing simple, straightforward Darboux transformation based on the Lax pair for Hirota equation, a higher-order nonlinear Schrödinger (HNLS) equation. As examples, one- and two-soliton solutions in explicit forms are given and their properties are also analyzed. A bound solution without interaction will be theoretically predicted if one can adjust frequency shift for each soliton appropriately. Further, we obtain the approximate eigenvalues by employing two-soliton solution and discuss analytically the interaction between neighboring solitons under the influence of the higher-order effects. It is shown that the combined effects of the higher-order effects can restrain the interaction between neighboring solitons to some extent. The results are proved by directly solving HNLS equation numerically.     

激光调Q CAI

从激光调Q速率方程出发,分析推导了激光调Q过程中,腔内光子数与工作物质内反转粒子数间的关系.再根据激光调Q的过程中,对腔Q值控制方式不同,将激光调Q技术分为:转镜调Q、声光调Q、电光调Q、饱和吸收调Q和脉冲透射式调Q.用C语言进行了激光调Q CAI软件的开发研制,获得了将文学、图形、动画和计算融为一体的,直观而生动地将调Q的理论和过程再现于屏幕的CAI课件.介绍了课件的内容、结构及其特点.     

漫谈金属探测器

提起金属探测器（Metal Detector），人们就会联想到探雷器，工兵用它来探测掩埋的地雷．金属探测器是一种专门用来探测金属的仪器，除了用于探测有金属外壳或金属部件的地雷之外，还可以用来探测隐蔽在墙壁内的电线、埋在地下的水管和电缆，甚至能够地下探宝，发现埋藏在地下的金属物体．目前还广泛应用于各种大型会议中心、会展场馆、体育场馆、公检法、监狱系统及娱乐场所的安全检查和工厂企业的防偷窃检查，甚至用于对高考禁带物品的检查．本文就来谈谈金属探测器的发展历史和工作原弹．     

第I种非对称三态叠加多模叠加态光场的高次和压缩——第一正交分量的N次方H压缩效应

构造了第孙中禹种强度不等的非对称三态叠加多模叠加态光场|ψ₁^(ABC)〉_q.利用多模压缩态理论研究了态|ψ₁^(ABC)〉_q第一正交分量高次和压缩.结果发现:①当构成态|ψ₁^(ABC)〉_q的三个多模相干态光场的强度不相等时,在一定条件下,态|ψ₁^(ABC)〉_q的第一正交分量可出现任意幂次的高次和压缩.②当上述的三个多模相干态光场强度相等时,态|ψ₁^(ABC)〉_q的第一正交分量的高次和压缩现象消失.在这种情况下,态|ψ₁^(ABC)〉_q的第一正交分量恒处于NH最小测不准态.     

酸糖蛋白手性柱分离6种手性化合物

通过考察缓冲液种类、浓度及其pH值对对映体在手性柱上的保留和分离行为的影响,以及流动相中加入不同种类、不同浓度的不带电荷的有机溶剂乙腈、甲醇、正丙醇、异丙醇、流动相流速和柱温对对映体分离能力的影响,优化了含碳手性中心的碱性药物苯丙哌林、酸性化合物MT-A5及MT-酸和含硫手性中心的质子泵抑制剂奥美拉唑、泮托拉唑、雷贝拉唑对映体分离条件,最佳手性分离条件为:苯丙哌林,0.05 mol/L磷酸二氢铵缓冲液(pH 3.0)-乙腈(95∶5,V/V)为流动相,流速为0.7 mL/m in,柱温为20℃;MT-A5及MT-酸,流动相为0.01 mol/L醋酸铵缓冲液(pH 5.0)-乙腈(74∶26,V/V),流速为0.9 mL/m in,柱温为20℃;泮托拉唑,流动相为10 mmol/L醋酸铵缓冲液(pH 5.5)-乙腈(93∶7,V/V),流速为0.9 mL/m in;柱温为20℃;奥美拉唑和雷贝拉唑,流动相为0.01 mol/L醋酸铵缓冲液(pH 3.0)-乙腈(95∶5,V/V),流速为0.7 mL/m in,柱温为20℃。实现了应用高效液相色谱法在α1-酸糖蛋白手性柱上对上述化合物的对映体分离,并成功用于手性药物合成中的对映体过量百分率的测定。     

Simplified analytic solutions and a novel fast algorithm for Yb-doped double-clad fiber lasers

In this paper, continuous wave Yb³⁺-doped double-clad fiber lasers (DCFLs) with linear-cavity are investigated theoretically and numerically using the rate equations. Under the steady state conditions, the simplified analytic solutions of Yb³⁺-doped DCFLs under considering the scattering loss are deduced in the strongly pump condition. Compared with the known analytic solutions in published literatures, our analytic solutions are more accurate, especially, at higher reflectivity of output mirror. In addition, a fast and stable algorithm based on the Newton-Raphson method is proposed to simulate numerically Yb³⁺-doped DCFLs. The results by simplified analytic solutions are in good agreement with those by the numerical simulation. Moreover, we have performed the optimization of an Yb³⁺-doped DCFL using the simplified analytic solutions and the numerical simulations, respectively.     

Bifurcations of Invariant Tori and Subharmonic Solutions of Singularly Perturbed System

This paper deals with bifurcations of subharmonic solutions and invariant tori generated from limit cycles in the fast dynamics for a nonautonomous singularly perturbed system. Based on Poincare map, a series of blow-up transformations and the theory of integral manifold, the conditions for the existence of invariant tori are obtained, and the saddle-node bifurcations of subharmonic solutions are studied.