Jet impingement cooling of a discretely heated portion of a protruding pedestal with a single round air jet

The influence of a protruding pedestal on impinging jet heat transfer is investigated. A discretely heated portion of a protruding pedestal is exposed to a single circular impinging air jet with Re=10,000–30,000. Jet exit diameters of 3.5, 9.5 and 21 mm are positioned at jet exit-to-surface distances of 2–5 diameters. The nondimensional heat transfer over the discretely heated portion of the pedestal is compared to a flat plate design to gauge the effects of Reynolds number, jet diameter and jet exit-surface spacing. In all cases, the presence of the protruding pedestal downstream is found to increase heat transfer.     

Diagonal quasi-Newton methods via least change updating principle with weighted Frobenius norm

Numerical Algorithms - This paper presents a class of low memory quasi-Newton methods with standard backtracking line search for large-scale unconstrained minimization. The methods are derived by...     

Accelerating the electron transfer of choline oxidase using ionic-liquid/NH2-MWCNTs nano-composite

A nano-composite consisting of amine functionalized multi-walled carbon nanotubes and a room temperature ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate) was prepared and used for modification of glassy carbon electrode. By immobilizing choline oxidase (ChOx) on the modified electrode, the enzyme direct electron transfer has been achieved. The modified electrode exhibited a pair of well-defined cyclic voltammetric peaks at a formal potential of ?0.395?V versus Ag/AgCl in 0.2?M phosphate buffer solution at pH 7.0. This peak was characteristic of ChOx-FAD/FADH₂ redox couple. The electrochemical parameters such as charge transfer coefficient (??) and apparent heterogeneous electron transfer rate constant (k _s) were estimated to be 0.36 and 2.74?s^?1, respectively. When the enzyme electrode was examined for the detection of choline, a relatively high sensitivity (2.59???A?mM^?1) was obtained. Under the optimized experimental conditions, choline was detected in the concentration range from 6.9?×?10^?3 to 6.7?×?10^?1?mM with a detection limit of 2.7???M. The peak currents of ChOx were reasonably stable and retained 90% of its initial current after a period of 2?months.     

Optimal inspection of a complex system subject to periodic and opportunistic inspections and preventive replacements

This paper proposes two optimization models for the periodic inspection of a system with “hard-type” and “soft-type” components. Given that the failures of hard-type components are self-announcing, the component is instantly repaired or replaced, but the failures of soft-type components can only be detected at inspections. A system can operate with a soft failure, but its performance may be reduced. Although a system may be periodically inspected, a hard failure creates an opportunity for additional inspection (opportunistic inspection) of all soft-type components. Two optimization models are discussed in the paper. In the first, soft-type components undergo both periodic and opportunistic inspections to detect possible failures. In the second, hard-type components undergo periodic inspections and are preventively replaced depending on their condition at inspection. Soft-type and hard-type components are either minimally repaired or replaced when they fail. Minimal repair or replacement depends on the state of a component at failure; this, in turn, depends on its age. The paper formulates objective functions for the two models and derives recursive equations for their required expected values. It develops a simulation algorithm to calculate these expected values for a complex model. Several examples are used to illustrate the models and the calculations. The data used in the examples are adapted from a real case study of a hospital’s maintenance data for a general infusion pump.     

Taming Griffiths' Singularities in Long Range Random Ising Models

We study long range random Ising models and develop modified high temperature and strong magnetic field expansions that give decay of truncated correlation functions and uniqueness of Gibbs states, in spite of the presence of Griffiths' singularities. Received: 30 September 1996 / Accepted: 18 February 1997     

Effect of hydrophilicity of room temperature ionic liquids on the electrochemical and electrocatalytic behaviour of choline oxidase

In the present report, six different nano-composites contaning the same amine functionalized multi-walled carbon nanotubes (NH(2)-MWCNTs) but different room temperature ionic liquids (RTILs) were prepared. Then, the efficiency of these nano-composites as supporting materials for studying the electrochemistry and electrocatalysis of choline oxidase (ChOx) as a model enzyme were compared. The corresponding cyclic voltammetric and amperometric data showed that the electrocatalytic activity and the electroanalytical performance of immobilized ChOx depends on the degree of hydrophilicity of RTILs used in the applied nano-composite. The higher stability (180 days), higher enzyme loading (6.56 mol cm(-2)), lower detection limit (3.85 μM) and wider linear range (0.005-0.8 mM) was obtained for the most hydrophilic RTIL (1-allyl-3-methylimidazolium bromide).     

Curcumin reduces α-synuclein induced cytotoxicity in Parkinson's disease cell model

Background  

Overexpression and abnormal accumulation of aggregated α-synuclein (αS) have been linked to Parkinson's disease (PD) and other synucleinopathies. αS can misfold and adopt a variety of morphologies but recent studies implicate oligomeric forms as the most cytotoxic species. Both genetic mutations and chronic exposure to neurotoxins increase αS aggregation and intracellular reactive oxygen species (ROS), leading to mitochondrial dysfunction and oxidative damage in PD cell models.     

Synthesis and Characterisation of Self‐Assembled and Self‐Adjuvanting Asymmetric Multi‐Epitope Lipopeptides of Ovalbumin

Designing a lipopeptide (LP) vaccine with a specific asymmetric arrangement of epitopes may result in an improved display of antigens, increasing host‐cell recognition and immunogenicity. This study aimed to synthesise and characterise the physicochemical properties of a library of asymmetric LP‐based vaccine candidates that contained multiple CD4⁺ and CD8⁺ T‐cell epitopes from the model protein antigen, ovalbumin. These fully synthetic vaccine candidates were prepared by microwave‐assisted solid phase peptide synthesis. The C12 or C16 lipoamino acids were coupled to the N or C terminus of the OVA CD4 peptide epitope. The OVA CD4 LPs and OVA CD8 peptide constructs were then conjugated using azide–alkyne Huisgen cycloaddition to give multivalent synthetic vaccines. Physiochemical characterisation of these vaccines showed a tendency to self‐assemble in aqueous media. Changes in lipid length and position induced self‐assembly with significant changes to their morphology and secondary structure as shown by transmission electron microscopy and circular dichroism.     

Biodegradation of Alprazolam in Pharmaceutical Wastewater Using Mesoporous Nanoparticles-Adhered Pseudomonas stutzeri

The release of pharmaceutical wastewaters in the environment is of great concern due to the presence of persistent organic pollutants with toxic effects on environment and human health. Treatment of these wastewaters with microorganisms has gained increasing attention, as they can efficiently biodegrade and remove contaminants from the aqueous environments. In this respect, bacterial immobilization with inorganic nanoparticles provides a number of advantages, in terms of ease of processing, increased concentration of the pollutant in proximity of the cell surface, and long-term reusability. In the present study, MCM-41 mesoporous silica nanoparticles (MSN) were immobilized on a selected bacterial strain to remove alprazolam, a persistent pharmaceutical compound, from contaminated water. First, biodegrading microorganisms were collected from pharmaceutical wastewater, and Pseudomonas stutzeri was isolated as a bacterial strain showing high ability to tolerate and consume alprazolam as the only source for carbon and energy. Then, the ability of MSN-adhered Pseudomonas stutzeri bacteria was assessed to biodegrade alprazolam using quantitative HPLC analysis. The results indicated that after 20 days in optimum conditions, MSN-adhered bacterial cells achieved 96% biodegradation efficiency in comparison to the 87% biodegradation ability of Pseudomonas stutzeri freely suspended cells. Kinetic study showed that the degradation process obeys a first order reaction. In addition, the kinetic constants for the MSN-adhered bacteria were higher than those of the bacteria alone.