Surface modification of poly(dimethylsiloxane) microchannels

This review looks at the efforts that are being made to modify the surface of poly(dimethylsiloxane) (PDMS) microchannels, in order to enhance applicability in the field of microfluidics. Many surface modifications of PDMS have been performed for electrophoretic separations, but new modifications are being done for emerging applications such as heterogeneous immunoassays and cell-based bioassays. These new modification techniques are powerful because they impart biospecificity to the microchannel surfaces and reduce protein adsorption. Most of these applications require the use of aqueous or polar solvents, which makes surface modification a very important topic.     

An ideal way of obtaining an optimal inspection permutation for a system with components connected in series

The problem of an inspection permutation or inspection strategy (first discussed in a research paper in 1989 and reviewed in another research paper in 1991) is revisited. The problem deals with an N‐component system whose times to failure are independent but not identically distributed random variables. Each of the failure times follows an exponential distribution. The components in the system are connected in series such that the failure of at least one component entails the failure of the system. Upon system failure, the components are inspected one after another in a hierarchical way (called an inspection permutation) until the component causing the system to fail is identified. The inspection of each component is a process that takes a non‐negligible amount of time and is performed at a cost. Once the faulty component is identified, it is repaired at a cost, and the repair process takes some time. After the repair, the system is good as new and is put back in operation. The inspection permutation that results in the maximum long run average net income per unit of time (for the undiscounted case) or maximum total discounted net income per unit of time (for the discounted case) is called the optimal inspection permutation/strategy. A way of determining an optimal inspection permutation in an easier fashion, taking advantage of the improvements in computer software, is proffered. Mathematica is used to showcase how the method works with the aid of a numerical example. Copyright © 2016 John Wiley & Sons, Ltd.     

Chip-based microfluidic devices coupled with electrospray ionization-mass spectrometry

We present the current status of the development of microfluidic devices fabricated on different substrates for coupling with electrospray ionization-mass spectrometry (ESI-MS). Until now, much success has been gained in fabricating the ESI chips, which show better performances due to miniaturization when compared with traditional methods. Integration of multiple steps for sample preparation and ESI sample introduction, however, remains a great challenge. This review covers the main technical development of electrospray device that were published from 1997 to 2004. This article does not attempt to be exclusive. Instead, it focuses on the publications that illustrated the breath of the development and applications of microchip devices for MS-based analysis.     

Electrophoretic mobility shift assay on poly(ethylene glycol)-modified glass microchips for the study of estrogen responsive element binding

The binding of estrogen receptor (ER) to estrogen response element (ERE) is essential for genomic pathways of estrogens and gel-based electrophoretic mobility shift assay (EMSA) is commonly used for analyzing ERE binding. Gel-based EMSA, however, requires the use of hazard radio isotopes and they are slow, labor-intensive and difficult to quantify. Here, we present quantitative affinity assays based on microchip electrophoresis using PEG-modified glass microchannels, which bear neutral surfaces against the adsorption of acidic DNA molecules and basic ER proteins. We first demonstrated the feasibility of the method by measuring binding constants of recombinant ERalpha and ERbeta with a consensus ERE sequence (cERE, 5'-GGTCAGAGTGACC-3') as well as with an ERE-like sequence (ERE 1576, 5'-GACCGGTCAGCGGACTCAC-3'). Changes in mobility as a function of protein-DNA molar ratios were plotted and the dissociation constants were determined based on non-linear curve fitting. The minimum amount of ER proteins required for one assay was around 0.2 ng and the run time for one chip analysis was less than 2 min. We further measured the estrogenic compound-mediated dissociation constants with recombinant ER proteins as well as with the extracted ERbeta from treated and untreated A549 bronchioloalveolar carcinoma cells. Dissociation constants determined by this method agree with the fact that agonist compounds such as 17beta-estradiol (1.70 nM), diethylstilbestrol (0.14 nM), and genistein (0.80 nM) assist ERE binding by decreasing the constants; while antagonist compounds such as testosterone (140.4 nM) and 4-hydroxytamoxifen (10.5 nM) suppress the binding by increasing the dissociation constant.     

Optimal scheduling of inspection times in a production process with a finite planning horizon

Inspection models applicable to a finite planning horizon are developed for the following lifetime distributions: uniform, exponential, and Weibull distribution. For a given lifetime distribution, maximization of profit is used as the sole optimization criterion for determining an optimal planning horizon over which a system may be operated as well as ideal inspection times. Illustrative examples (focusing on the uniform and Weibull distributions and using Mathematica programs) are given. For some situations, evenly spreading inspections over the entire planning horizon are seen to result in the attainment of desirable profit levels over a shorter planning horizon. Scope for further research is given as well. Copyright © 2016 John Wiley & Sons, Ltd.     

Photopatterning of tough single-walled carbon nanotube composites in microfluidic channels and their application in gel-free separations

We report on the photopatterning of single carbon nanotube composites with soft hydrogel polymers in glass microchannels. Since the hydrogels by themselves are able to withstand liquid flow within the microchannels, we covalently combined them with single-walled carbon nanotubes to impart mechanical strength. We attempted this approach by patterning the gels within the microchannels without prior surface modifications. Our results show that the 1-cm nanocomposite hydrogels are far stronger than the free hydrogels. Moreover, the nanocomposites were able to concentrate and separate proteins within a 1.5-cm distance using gel-free buffers. The separation cannot only be tuned by changing the running buffer; the lack of gels in the running buffer reduces the chance of channel blockage and thus the lifetime of the device is prolonged. The usefulness of the patterned nanocomposites may be extended by a wide selection of nanocomposite properties and monomers to find a broad range of applications in lab-on-chip technology.