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排序方式: 共有241条查询结果,搜索用时 15 毫秒
1.
Josephson effects have been observed in bulk samples of Y-Ba-Cu-O. The magnitude of the zero-voltage current is found to change
systematically with externally applied small magnetic fields of a few mG. It is also found to vary when samples are irradiated
with microwaves. These observations suggest the presence of inter-grain Josephson junctions. 相似文献
2.
Tobacco protein separation by aqueous two-phase extraction 总被引:13,自引:0,他引:13
Tobacco has long been considered as a host to produce large quantity of high-valued recombinant proteins. However, dealing with large quantities of biomass is a challenge for downstream processing. Aqueous two-phase extraction (ATPE) has been widely used in purifying proteins from various sources. It is a protein-friendly process and can be scaled up easily. In this paper, ATPE was studied for its applicability to recombinant protein purification from tobacco with egg white lysozyme as the model protein. Separate experiments with poly(ethylene glycol) (PEG)-salt-tobacco extract and PEG-salt-lysozyme were carried out to determine the partition behavior of tobacco protein and lysozyme, respectively. Two-level fractional factorial designs were used to study the effects of factors such as, PEG molecular mass, PEG concentration, the concentration of phase forming salt, sodium chloride concentration and pH, on protein partitioning. The results showed that, among the studied systems, PEG-sodium sulfate system was most suitable for lysozyme purification. Detailed experiments were conducted by spiking lysozyme into the tobacco extract. The conditions with highest selectivity of lysozyme over native tobacco protein were determined using a response surface design. The purification factor was further improved by decreasing the phase ratio along the tie line corresponding to the phase compositions with the highest selectivity. Under selected conditions the lysozyme yield was predicted to be 87% with a purification factor of 4 and concentration factor of 14. From this study, ATPE was shown to be suitable for initial protein recovery and partial purification from transgenic tobacco. 相似文献
3.
The reaction between chloramphenicol and chloramine-T, chloramine-B, bromamine-B or bromamine-T has been found to proceed quantitatively over a wide range of experimental conditions. Simple titrimetric procedures for the estimation of chloramphenicol with N-haloamines have been developed. Oxidation of the antibiotic involves a four-electron change and the products of oxidation have been identified. The methods are useful for the estimation of this antibiotic in medicinal formulations. 相似文献
4.
Formation of a thin polyaniline film on hydrous zirconia (ZrO2) surface was carried out using adsorbed surfactant bilayers on ZrO2 as reaction sites. Aniline was adsolubilized in hexadecyltrimethylammonium bromide (HDTAB) admicelles formed on the surface of ZrO2 by adsorption. Subsequent polymerization of the adsolubilized aniline monomer showed effective conversion of aniline to polyaniline. The formation of the polyaniline coated ZrO2 has been confirmed by UV-visible spectroscopy, FT-IR spectroscopy, and conductivity measurements. Various parameters involved during the adsorption process were studied. Selection of pH 9.0 as the pH of all experimental feed solutions was governed by the knowledge of point of zero charge (PZC) of ZrO2. Effect of aniline concentration on HDTAB adsorption was studied and it was observed that increase in aniline concentration decreased the amount of HDTAB adsorbed on ZrO2. Addition of salt (0.05 M NaCl) in the feed solution increased HDTAB adsorption and drastically reduced the effect of aniline concentration on HDTAB adsorption. 相似文献
5.
Jayaram B McConnell K Dixit SB Das A Beveridge DL 《Journal of computational chemistry》2002,23(1):1-14
Noncovalent association of proteins to specific target sites on DNA--a process central to gene expression and regulation--has thus far proven to be idiosyncratic and elusive to generalizations on the nature of the driving forces. The spate of structural information on protein--DNA complexes sets the stage for theoretical investigations on the molecular thermodynamics of binding aimed at identifying forces responsible for specific macromolecular recognition. Computation of absolute binding free energies for systems of this complexity transiting from structural information is a stupendous task. Adopting some recent progresses in treating atomic level interactions in proteins and nucleic acids including solvent and salt effects, we have put together an energy component methodology cast in a phenomenological mode and amenable to systematic improvements and developed a computational first atlas of the free energy contributors to binding in approximately 40 protein-DNA complexes representing a variety of structural motifs and functions. Illustrating vividly the compensatory nature of the free energy components contributing to the energetics of recognition for attaining optimal binding, our results highlight unambiguously the roles played by packing, electrostatics including hydrogen bonds, ion and water release (cavitation) in protein-DNA binding. Cavitation and van der Waals contributions without exception favor complexation. The electrostatics is marginally unfavorable in a consensus view. Basic residues on the protein contribute favorably to binding despite the desolvation expense. The electrostatics arising from the acidic and neutral residues proves unfavorable to binding. An enveloping mode of binding to short stretches of DNA makes for a strong unfavorable net electrostatics but a highly favorable van der Waals and cavitation contribution. Thus, noncovalent protein-DNA association is a system-specific fine balancing act of these diverse competing forces. With the advances in computational methods as applied to macromolecular recognition, the challenge now seems to be to correlate the differential (initial vs. final) energetics to substituent effects in drug design and to move from affinity to specificity. 相似文献
6.
Monte Carlo computer simulations were performed on dilute aqueous solutions of the dimethylphosphate anion and the sodium dimethylphosphate ion pair, with the two phosphodiester torsional angles in the gauche–gauche, gauche–trans, and trans–trans conformations. The structural and energetic aspects of the aqueous hydration of each molecule were analyzed in terms of quasi component distribution functions based on the proximity criterion and partitioned into ionic, hydrophilic, and hydrophobic contributions to facilitate an understanding of the hydration pattern and conformational trends in these multifunctional solutes. Special attention was also paid to methodological issues affecting hydration, such as statistical uncertainty in the determined hydration indices, choice of partial atomic charges for the solute atoms, and solute–water interaction potentials adopted in the simulations. The results showed that gauche–trans and gauche–gauche forms are equally favorable for the dimethylphosphate anion with the trans extended form destabilized by hydration. The sodium dimethylphosphate ion pair hydration energetically favors the trans–trans conformation. 相似文献
7.
J. P. Dauer K. Balachandran P. Balasubramaniam 《Journal of Optimization Theory and Applications》1994,83(1):167-179
In this paper, sufficient conditions are obtained for the asymptotic null controllability of the system $$\dot x(t) = g(t,x(t)) + B(t,x(t))u(t) + f(t,x(t),u(t)).$$ The results are obtained by using the Leray-Schauder fixed-point theorem. 相似文献
8.
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10.
This paper investigates the delay-probability-distribution-dependent stability problem of uncertain stochastic genetic regulatory networks (SGRNs) with mixed time-varying delays. The information of the probability distribution of the time-delay is considered and transformed into parameter matrices of the transferred SGRNs model. Based on the Lyapunov–Krasovskii functional and stochastic analysis approach, a delay-probability-distribution-dependent sufficient condition is obtained in the linear matrix inequality (LMI) form such that delayed SGRNs are robustly globally asymptotically stable in the mean square for all admissible uncertainties. Finally a numerical example is given to illustrate the effectiveness of our theoretical results. 相似文献