AN ELECTRON MICROSCOPIC STUDY ON CELL REFORMATION OF CHIROCEPHALUS YOLK GRANULES CULTURED IN VITRO

The reformation of nuclei and cells on the basis of yolk granules in vitro has been examined under electron microscope by using the mature ooeytes of the middle female intersex of Chirocephalus nankinensis. And it has been shown that the forms of reformed nuclear and cellular structures vary, which may be related to the developmental stages of the middle female intersex, types of reforming process, culturing times as well as internal and external conditions of yolk granules. We have also studied the structural changes of yolk granules and some transition forms during the reformation of nuclei and cells. The results are fairly in agreement with those of our previous reports, i. e. the time-lapse microcinematographic and phase contrast studies on the cell reformation of yollk granules in vitro and the electron microscopic investigations on the cell reformation of yolk granules of mature oocytes in situ.     

南京丰年虫卵黄颗粒在离体培养下重建细胞的电子显微镜观察

本文主要叙述南京丰年虫中雌中间性以卵黄颗粒为基础在离体培养下重建核和细胞的电镜观察。观察到的核结构和细胞结构的形式是多种多样的,这可能与中雌的发育情况,不同方式的重建过程、不同培养时间以及不同发展程度有关。同时也观察到卵黄颗粒结构的变化,核重建和细胞重建的某些过渡形态等。这些结果与中雌以卵黄颗粒为基础在离体培养下重建细胞的显微缩时电影和相差定位观察是可以相互对应的,而且与中雌生殖囊内长成卵母细胞以卵黄颗粒为基础重建细胞从原位切片进行电子显微镜观察的结果也是相类似的。     

TIME-LAPSE MICROCINEMATOGRAPHIC AND PHASE CONTRAST STUDIES ON THE CELL REFORMATION OF CHIROCEPHALUS YOLK GRANULES IN VITRO

In this article we report on a time-lapse microcinematographic and phase contrast study of the cell reformation of yolk granules which were isolated from mature oocytes in the genital sac of the middle female intersex of Chirocephalus nankinensis. The observations on yolk granules were made in fixed positions and the morphological changes of cell reformation were continuously recorded in vitro.The transformation of yolk granules into cellular structures may be tentatively described as follows: At the early stages in culture, it can be seen that the granular or bubbly structures gradually appear within the hyaloid and homogeneous yolk granules; the appearance of yolk granules gradually changes from elliptical into round shape. With further transformation, their appearances in general take the shape of oblate spheroids, their size is smaller than before and inside the yolk granules their granular and bubbly structures become more and more obvious. Finally, the nuclear and cellular structures can be seen i     

南京丰年虫卵黄颗粒在离体培养下重建为细胞的显微缩时电影和相差定位观察

本文就南京丰年虫(Chirocephalus nankinensis) 中雌中间性(middle female intersex)生殖囊内长成卵母细胞的卵黄颗粒,在离体培养下,借助于显微缩时电影和相差定位观察,叙述了细胞重建(cell reformation)的形态变化。 由卵黄颗粒转变为细胞结构的过程,大致可叙述如下:培养早期,在透明、均匀的卵黄颗粒中,逐渐出现颗粒状或小泡状结构,卵黄颗粒外形由椭圆形逐渐变圆;在进一步转变中,外形一般已成为扁圆形,体积较前变小,内部颗粒状和泡状结构更为显著,接着可见核结构和细胞结构。通过显微缩时电影和相差定位观察,连续地描述了从长成卵母细胞流出来的、外面带有卵母细胞胞浆(细胞质)的单个卵黄颗粒,在离体培养下,通过自组织、自装配(自组装),可以一步一步地建成细胞结构。现在,已经可以大批地培养出这种细胞结构,而且进行了多次的重复。这样,就能真实地反映了细胞重建;从形态变化看,它是客观存在的现象。     

《抛物线的标准方程》呈现方式的一种新尝试

1教学内容的地位、作用及学情分析 《普通高中数学课程标准（实验）》指出：数学探究、数学建模、数学文化是贯穿于整个高中数学课程中的．数学探究是指学生围绕某个数学问题,自主探究、学习的过程．这个过程包括：观察分析数学事实,