小麦花粉植株的诱导及其后代的观察

用补加生长素、加或不加激动索的Ms培养基培养普通小麦(Triticum aestivum)的花药,成功地诱导小麦花粉通过形成愈伤组织发育为完整植株。研究表明:处于单核中期阶段的花粉最适合于诱导形成愈伤组织;补加水解乳蛋白和适当提高蔗糖浓度,对促进花粉形成愈伤组织有一定效果。用不同材料(品种或杂交组合)的花药进行培养,结果有较大差异。杂种花粉植株的种子后代表现整齐一致,没有分离,说明将这种方法用于育种是有希望的。     

用花粉管途径获得小麦转基因植株

本文将带GUS标记基因的质粒pBI121用花粉管途径转化普通小麦(T. aestivum L)品种小山3号的小花。从结实的106粒种子中,经点杂交初步筛选,再经Southern分子杂交鉴定,选出5株转基因小麦,转化率为4.7％。同时用荧光法和X-Gluc染色法测试,检测到这些植株中有GUS基因的表达产物β-葡糖苷酸酶(GUS)存在,证明GUS基因已整合到小麦植株中,并能在植物体中表达。     

Transgenic Wheat Plants Obtained With Pollen-Tube Pathway Method

β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.