A new method to determine viscosity of liquids using vibration principles

A new method for determining viscosity of liquids is examined. The method employs the principles of vibration and measures the viscous damping due to the motion of a liquid placed in a cylindrical tube. The apparatus and the test liquid are treated as a dynamic system and the measured mechanical impedances are used to calculate energy dissipation due to the viscous damping. The newly designed apparatus is able to generate shear deformations in the liquid without using moving solid surfaces. A harmonic varying force with a frequency close to the resonance frequency of the system is applied through a piston and the resulting velocities of the oscillations generated in the system are measured. Liquids with higher viscosities result in lower velocities due to the higher damping. Analytical equations are provided to relate the viscous damping of the dynamic system to the viscosity of the liquids. The viscosities obtained from the proposed method are in good agreement with the ones obtained from standard rotational viscometry using a cone and plate geometry.     

A new model for the study of rain-wind-induced vibrations of a simple oscillator

In this paper, a model equation is presented for the study of rain-wind-induced vibrations of a simple oscillator. As will be shown the presence of raindrops in the wind-field may have an essential influence on the dynamic stability of the oscillator. In this model equation the influence of the variation of the mass of the oscillator due to an incoming flow of raindrops hitting the oscillator and a mass flow which is blown and shaken off is investigated. The time-varying mass is modeled by a time harmonic function whereas simultaneously also time-varying lift and drag forces are considered.     

Piecewise-linear restoring force surfaces for semi-nonparametric identification of nonlinear systems

A method for identifying a piecewise-linear approximation to the nonlinear forces acting on a system is presented and demonstrated using response data from a micro-cantilever beam. It is based on the Restoring Force Surface (RFS) method by Masri and Caughey, which is very attractive when initially testing a nonlinear system because it does not require the user to postulate a form for the nonlinearity a priori. The piecewise-linear fitting method presented here assures that a continuous piecewise-linear surface is identified, is effective even when the data does not cover the phase plane uniformly, and is more computationally efficient than classical polynomial based methods. A strategy for applying the method in polar form to sinusoidally excited response data is also presented. The method is demonstrated on simulated response data from a cantilever beam with a nonlinear electrostatic force, which highlights some of the differences between the local, piecewise-linear model presented here and polynomial-based models. The proposed methods are then applied to identify the force-state relationship for a micro-cantilever beam, whose response to single frequency excitation, measured with a Laser Doppler Vibrometer, contains a multitude of harmonics. The measurements suggest that an oscillatory nonlinear force acts on the cantilever when its tip velocity is near maximum during each cycle.     

Controlling orientations of immobilized oligopeptides using N-terminal cysteine labels

This letter reports a strategy of using N-terminal cysteine labels for controlling the immobilization of oligopeptides on aldehyde-terminated surfaces through the formation of stable thiazolidine rings. We also study the effect of cysteine position (either N-terminal or C-terminal) and lysine residue on the immobilization of oligopeptides. On the basis of our ellipsometry and quartz crystal microbalance (QCM) results, we conclude that the proposed immobilization strategy is highly site-specific. It works only when cysteine is in the N-terminal position, and the formation of thiazolidine is much faster than the formation of imines between lysine residues and aldehydes, even in the presence of a reducing agent such as NaBH(3)CN. By labeling an oligopeptide CSNKTRIDEANNKATKML with an N-terminal cysteine, we immobilize this oligopeptide on an aldehyde-terminated surface and investigate the enzymatic activity of trypsin acting on the oligopeptide. It is found that trypsin is able to cleave the immobilized oligopeptide having a single anchoring point at the N-terminal cysteine. No cleavage is observed when the oligopeptide is immobilized through multiple anchoring points at lysine residues.     

Controlling and manipulating supported phospholipid monolayers as soft resist layers for fabricating chemically micropatterned surfaces

Chemically micropatterned surfaces have broad applications in many fields. In this paper, we report a new method for preparing chemically micropatterned surfaces by controlling and manipulating supported phospholipid monolayers as soft resist layers with molecular-level precision. First, we introduce self-assembled supported phospholipid monolayers on solid surfaces and use a microcontact lift-up process to create micropatterned phospholipid monolayers (with micrometer resolution) on the surface. Next, the micropatterned phospholipid monolayers can function as "soft" resist layers to protect underlying solid substrates and create either positive or negative chemically micropatterned surfaces during subsequent treatments. Unlike traditional "hard" resist layers which can only be removed by using harsh chemical treatments, this novel soft resist layer only comprises a single layer of compact phospholipid; therefore, it can be easily removed by water rinsing after the preparation of micropatterns. This method is also versatile. It can be applied to prepare a protein microarray or silver patterns on solid substrates.     

Detecting and differentiating Escherichia coli strain TOP10 using optical textures of liquid crystals

We report a method for detecting Escherichia coli using a nematic liquid crystal (LC), 4-cyano-4′-pentylbiphenyl (5CB). Among three E. coli strains tested, TOP10 strain grown on agar plates induces a homeotropic orientation of LCs whereas DH5α and JM109 strains do not. This results in a clear distinction in the optical appearance of LCs as either uniformly dark or bright under polarised light. The LC-based method provides a simple, rapid and low-cost method of identifying E. coli strains.     

On-chip measurements of cell compressibility via acoustic radiation

Measurements of mechanical properties of biological cells are of great importance because changes in these properties can be strongly associated with the progression of cell differentiation and cell diseases. Although state of the art methods, such as atomic force microscopy, optical tweezers and micropipette aspiration, have been widely used to measure the mechanical properties of biological cells, all these methods involve direct contact with the cell and the measurements could be affected by the contact or any local deformation. In addition, all these methods typically deduced the Young's modulus of the cells based on their measurements. Herein, we report a new method for fast and direct measurement of the compressibility or bulk modulus of various cell lines on a microchip. In this method, the whole cell is exposed to acoustic radiation force without any direct contact. The method exploits the formation of an acoustic standing wave within a straight microchannel. When the polystyrene beads and cells are introduced into the channel, the acoustic radiation force moves them to the acoustic pressure node and the movement speed is dependent on the compressibility. By fitting the experimental and theoretical trajectories of the beads and the cells, the compressibility of the cells can be obtained. We find that the compressibility of various cancer cells (MCF-7: 4.22 ± 0.19 × 10(-10) Pa(-1), HEPG2: 4.28 ± 0.12 × 10(-10) Pa(-1), HT-29: 4.04 ± 0.16 × 10(-10) Pa(-1)) is higher than that of normal breast cells (3.77 ± 0.09 × 10(-10) Pa(-1)) and fibroblast cells (3.78 ± 0.17 × 10(-10) Pa(-1)). This work demonstrates a novel acoustic-based method for on-chip measurements of cell compressibility, complementing existing methods for measuring the mechanical properties of biological cells.