Establishment of CRISPR/Cas9-Mediated Knock-in System for Porcine Cells with High Efficiency

Applied Biochemistry and Biotechnology - Since the birth of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9, the new genome engineering technology has become a hot topic in...     

A New Coumarin-Acridone Compound as a Fluorescence Probe for Fe3+ and Its Application in Living Cells and Zebrafish

A new coumarin-acridone fluorescent probe S was designed and synthesized, and the structure was confirmed with ¹H/¹³C NMR spectrometry, single-crystal X-ray diffraction, and high-resolution mass spectrometry. This probe has high sensitivity and selectivity for Fe³⁺ over other testing metal ions at 420 or 436 nm in acetonitrile–MOPS (3-Morpholinopropanesulfonic Acid) buffer solution (20.0 μM, pH = 6.9, 8:2 (v/v)). Under physiological conditions, the probe displayed satisfying time stability with a detection limit of 1.77 µM. In addition, probe S was successfully used to detect intracellular iron changes through a fluorescence-off mode, and the imaging results of cells and zebrafish confirmed their low cytotoxicity and satisfactory cell membrane permeability, as well as their potential biological applications.     

MECHANICAL ANALYSIS ON THE COLLAPSE ACCIDENT OF BUS SHELTER CEILINGS AFTER SNOWSTORM 1)

2018年1月4日,合肥市普降暴雪,望江路沿线多座公交候车亭顶棚坍塌,造成多人受伤1人死亡的严重后果。本文首先通过合理简化,建立了候车亭棚体受力模型;然后,利用材料力学组合变形理论,对棚体的立柱、 连接件和顶棚开展了受力分析,指出了连接件和立柱受到较大弯矩,为候车亭棚体中需要加强支护的部位;最后,通过事故分析,揭示了连接件尺寸过小和强度不足是导致此次顶棚坍塌事故的直接原因,亦说明了设计缺陷和施工质量不足是造成本次坍塌的根本所在。研究结论,对公交候车亭的优化设计、施工和安全管理具有非常重要的指导意义,为避免此类坍塌事故的再次发生提供了重要的借鉴。