DETERMINATION OF THE KINETICS OF THE BACK REACTION OF PHOTOSYSTEM II IN THE PRESENCE OF 3–(3,4-DICHLORPHENYL)-1,1-DIMETHYLUREA FROM LUMINESCENCE MEASUREMENTS

Abstract— An alternative method to that used by Mar and Roy (1974) for the determination of the kinetics of the back reaction of photosystem II from the luminescence decay curve in the presence of 3–(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) has been suggested. The new theory relies upon two hypotheses: the well-known recombination hypothesis of luminescence and the assumption that the luminescence yield in the seconds region is given by the variable part of the live fluorescence yield. The second hypothesis was introduced since assuming a constant luminescence yield results in kinetic data that are not consistent with measurements of the kinetics of the back reaction by the restoration of the area over the fluorescence rise curve. The dependence of the live fluorescence yield from the concentration of closed PS II traps was assumed to be represented by Delosme's expression originally derived for the rise curve of the fluorescence yield in the presence of DCMU.
The theory is based on the fact that then the partial and total light sums of luminescence are simple functions of the concentration of the primary electron acceptor Q^- of PS II. Thus, after integrating the luminescence decay curve the theory permits a convenient evaluation of the kinetics of the back reaction [Q^-]( t ) in terms of the partial and total light sum.
This method was applied in order to determine the kinetics of the back reaction in Chlorella fusca in the presence of DCMU. It is shown that the kinetics of deactivation of the S, state can be described using the expression for the kinetics of the back reaction derived by Mar and Roy. As an alternative explanation, a biphasic first order decay of S₂ is proposed.     

Zur Luminiszenz von Cr(3)-Verbindungen

Whereas 2.4 is likely to be an upper limit for theDq/B-value of Cr(3)-complexes which show fluorescence the existence of a lower bound is rather unlikely. Experimental evidence suggests that the energy difference between the minima of the² E _g- and the⁴ T _2g-potential curves is one of the factors determining the relative intensities of phosphorescence and fluorescence.