Simultaneous Determination of Clobetasol Propionate and Calcipotriol in a Novel Fixed Dose Emulgel Formulation by LC-UV

A new, rapid, selective, cheap and simple RP-LC method has been developed and validated for the simultaneous determination of clobetasol propionate and calcipotriol mixtures in bulk drugs (raw materials) and in a novel-fixed dose emulgel formulation. Separation was carried out using a NovaPak C18 column with methanol:water (74:26 v/v) as mobile phase for isocratic elution at a flow rate of 1.0 mL min^?1. The column temperature was set at 25 °C. Calibration curves were established ranging between 0.5 and 20 μg mL^?1 and 0.5 and 10 μg mL^?1 for clobetasol propionate and calcipotriol, respectively. Limit of detection and limit of quantification values of the method was found as 0.16 and 0.48 μg mL^?1 for clobetasol propionate and 0.10 and 0.30 μg mL^?1 for calcipotriol, respectively. The method was validated in accordance with ICH guidelines and obtained results proved that the proposed method was precise, accurate, selective and sensitive for the simultaneous analysis of clobetasol propionate and calcipotriol. The proposed method can be easily applied for the simultaneous determination of clobetasol propionate and calcipotriol in prepared emulgel formulations. The obtained validation results showed that the RP-LC method is suitable for routine quantification of clobetasol propionate and calcipotriol in emulgel formulations with high precision and accuracy.     

Topical emulgel formulation containing inclusion complex of calcipotriol with cyclodextrin

Psoriasis is a relatively common, chronic, inflammatory disease that affects the skin, scalp, and joints. Calcipotriol is one of the most commonly used topical agents for the treatment of psoriasis. However, it is a water-insoluble active substance and frequently leads to skin irritation in patients. Cyclodextrins (CDs) are cyclic oligosaccharides consisting of (α-1,4-)-linked d-glucopyranose units. CD molecules have a hydrophilic outer surface and a lipophilic central cavity, and they are able to form inclusion complexes in aqueous solutions with many drugs. They can increase bioavailability, aqueous solubility, and stability and also reduce the side effects of the drugs. The aim of this study was to develop a new topical drug delivery system of calcipotriol in order to improve the solubility and dissolution characteristics of the drug and reduce the undesirable side effects. For this purpose, an inclusion complex of calcipotriol with Captisol^® was prepared, and complex formation was confirmed. The inclusion complex and pure drug were formulated separately in an emulgel base. Dissolution profiles of calcipotriol from emulgel formulations were compared with a commercial product of the drug. The drug release was significantly increased with the emulgel formulations compared to the commercial cream product.     

Tunning of Templated CuWO4 Nanorods Arrays Thickness to Improve Photoanode Water Splitting

The fabrication of the photoanode of the n-type CuWO₄ nanorod arrays was successfully carried out through electrochemical deposition using anodic aluminum oxide (AAO) control templates and for the first time produced distinct gaps between the nanorod arrays. The effectiveness and efficiency of the resulting deposition was shown by the performance of the photoelectrochemical (PEC) procedure with a current density of 1.02 mA cm⁻² with irradiation using standard AM 1.5G solar simulator and electron changed radiation of 0.72% with a bias potential of 0.71 V (vs. Ag/AgCl). The gap between each nanorod indicated an optimization of the electrolyte penetration on the interface, which resulted in the expansion of the current density as much as 0.5 × 10²⁴ cm⁻³ with a flat band potential of 0.14 V vs. Ag/AgCl and also a peak quantum efficiency of wavelength 410 nm. Thus, also indicating the gaps between the nanorod arrays is a promising structure to optimize the performance of the PEC water splitting procedure as a sustainable energy source.