CD47 in the Brain and Neurodegeneration: An Update on the Role in Neuroinflammatory Pathways

CD47 is a receptor belonging to the immunoglobulin (Ig) superfamily and broadly expressed on cell membranes. Through interactions with ligands such as SIRPα, TSP-1, integrins, and SH2-domain bearing protein tyrosine phosphatase substrate-1 (SHPS-1), CD47 regulates numerous functions like cell adhesion, proliferation, apoptosis, migration, homeostasis, and the immune system. In this aspect, previous research has shown that CD47 modulates phagocytosis via macrophages, the transmigration of neutrophils, and the activation of T-cells, dendritic cells, and B-cells. Moreover, several studies have reported the increased expression of the CD47 receptor in a variety of diseases, including acute lymphoblastic leukemia (ALL), chronic myeloid leukemia, non-Hodgkin’s lymphoma (NHL), multiple myeloma (MM), bladder cancer, acute myeloid leukemia (AML), Gaucher disease, Multiple Sclerosis and stroke among others. The ubiquitous expression of the CD47 cell receptor on most resident cells of the CNS has previously been established through different methodologies. However, there is little information concerning its precise functions in the development of different neurodegenerative pathologies in the CNS. Consequently, further research pertaining to the specific functions and roles of CD47 and SIRP is required prior to its exploitation as a druggable approach for the targeting of various neurodegenerative diseases that affect the human population. The present review attempts to summarize the role of both CD47 and SIRP and their therapeutic potential in neurodegenerative disorders.     

The critical role of channel cross-sectional area in microchannel flow boiling heat transfer

Experiments are conducted with a perfluorinated dielectric fluid, Fluorinert FC-77, to identify the critical geometric parameters that affect flow boiling heat transfer and flow patterns in microchannels. In recent work by the authors (Harirchian and Garimella, 2009), seven different silicon test pieces containing parallel microchannels of widths ranging from 100 to 5850 μm, all with a depth of 400 μm were tested and it was shown that for a fixed channel depth, the heat transfer coefficient was independent of channel width for microchannels of widths 400 μm and larger, with the flow regimes in these microchannels being similar; nucleate boiling was also found to be dominant over a wide range of heat fluxes. In the present study, experiments are performed with five additional microchannel test pieces with channel depths of 100 and 250 μm and widths ranging from 100 to 1000 μm. Flow visualizations are performed using a high-speed digital video camera to determine the flow regimes, with simultaneous local measurements of the heat transfer coefficient and pressure drop. The aim of the present study is to investigate as independent parameters the channel width and depth as well as the aspect ratio and cross-sectional area on boiling heat transfer in microchannels, based on an expanded database of experimental results. The flow visualizations and heat transfer results show that the channel cross-sectional area is the important governing parameter determining boiling mechanisms and heat transfer in microchannels. For channels with cross-sectional area exceeding a specific value, nucleate boiling is the dominant mechanism and the boiling heat transfer coefficient is independent of channel dimensions; below this threshold value of cross-sectional area, vapor confinement is observed in all channels at all heat fluxes, and the heat transfer rate increases as the microchannel cross-sectional area decreases before premature dryout occurs due to channel confinement.     

Effects of channel dimension,heat flux,and mass flux on flow boiling regimes in microchannels

Experiments are conducted with a perfluorinated dielectric fluid, Fluorinert FC-77, to investigate the effects of channel size and mass flux (225–1420 kg/m²s) on microchannel flow boiling regimes by means of high-speed photography. Seven different silicon test pieces with parallel microchannels of widths ranging from 100 to 5850 μm, all with a depth of 400 μm, are considered. Flow visualizations are performed with a high-speed digital video camera while local measurements of the heat transfer coefficient are simultaneously obtained. The visualizations and the heat transfer data show that flow regimes in the microchannels of width 400 μm and larger are similar, with nucleate boiling being dominant in these channels over a wide range of heat flux. In contrast, flow regimes in the smaller microchannels are different and bubble nucleation at the walls is suppressed at a relatively low heat flux for these sizes. Two types of flow regime maps are developed and the effects of channel width on the flow regime transitions are discussed.     

Effects of 3D electrodes arrangement in a novel AC electroosmotic micropump: Numerical modeling and experimental validation

To date, a comprehensive systematic optimization framework, capable of accurately predicting an efficient electrode geometry, is not available. Here, different geometries, including 3D step electrodes, have been designed in order to fabricate AC electroosmosis micropumps. It is essential to optimize both geometrical parameters of electrode, such as width and height of steps on each base electrode and their location in one pair, the size of each base electrode (symmetric or asymmetric), the gap of electrode pairs, and nongeometrical parameters such as fluid flow in a channel and electrical characteristics (e.g., frequency and voltage). The governing equations comprising of electric domain and fluid domain have been coupled using finite element method. The developed model was employed to investigate the effect of electrode geometric parameters on electroosmotic slip velocity and its subsequent effect on pressure and flow rate. Numerical simulation indicates that the optimal performance can be achieved using a design with varying step height and displacement, at a given voltage (2.5 V) and frequency (1 kHz). Finally, in order to validate the numerical simulation, the optimal microchip was fabricated using a combination of photolithography, electroplating, and a polydimethylsiloxane microchannel. Our results indicate that our micropump is capable of generating a pressure, velocity, and flow rate of 74.2 Pa, 1.76 mm/s, and 14.8 µl/min, respectively. This result reveals that our proposed geometry outperforms the state-of-the-art micropumps previously reported in the literature by improving the fluid velocity by 32%, with 80% less electrodes per unit length, and whereas the channel length is ∼80% shorter.