排序方式: 共有24条查询结果,搜索用时 171 毫秒
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Stopar D Strancar J Spruijt RB Hemminga MA 《Journal of chemical information and modeling》2005,45(6):1621-1627
Molecular modeling based on a hybrid evolutionary optimization and an information condensation algorithm, called GHOST, of spin label ESR spectra was applied to study the structure and dynamics of membrane proteins. The new method is capable of providing detailed molecular information about the conformational space of the spin-labeled segment of the protein in a membrane system. The method is applied to spin-labeled bacteriophage M13 major coat protein, which is used as a model membrane protein. Single cysteine mutants of the coat protein were labeled with nitroxide spin labels and incorporated in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayers. The new computational method allows us to monitor distributions of local spatial constraints and molecular mobility, in addition to information about the location of the protein in a membrane. Furthermore, the results suggest that different local conformations may coexist in the membrane protein. The knowledge of different local conformations may help us to better understand the function-structure relationship of membrane proteins. 相似文献
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A.M.J. Spruijt 《Solid State Communications》1973,13(11):1919-1922
In a planar oriented layer of a nematic liquid crystal regions of differing chirality, separated by a twist-disclination line, are observed. At least four modifications of this disclination are observed in a parallel layer (zero twist) and three in a twisted layer. The nature of these modified twist-disclinations is described. 相似文献
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Dóka O Ficzek G Bicanic D Spruijt R Luterotti S Tóth M Buijnsters JG Végvári G 《Talanta》2011,84(2):341-346
The analytical performance of the newly proposed laser-based photoacoustic spectroscopy (PAS) and of optothermal window (OW) method for quantification of total anthocyanin concentration (TAC) in five sour cherry varieties is compared to that of the spectrophotometry (SP). High performance liquid chromatography (HPLC) was used to identify and quantify specific anthocyanins. Both, PAS and OW are direct methods that unlike SP and HPLC obviate the need for the extraction of analyte. The outcome of the study leads to the conclusion that PAS and OW are both suitable for quick screening of TAC in sour cherries. The correlation between the two methods and SP is linear with R2 = 0.9887 for PAS and R2 = 0.9918 for OW, respectively. Both methods are capable of the rapid determination of TAC in sour cherries without a need for a laborious sample pretreatment. 相似文献
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M Lemmers E Spruijt S Akerboom IK Voets AC van Aelst MA Cohen Stuart J van der Gucht 《Langmuir : the ACS journal of surfaces and colloids》2012,28(33):12311-12318
We have prepared an aqueous physical gel consisting of negatively charged silica nanoparticles bridged by ABA triblock copolymers, in which the A blocks are positively charged and the B block is neutral and water-soluble. Irreversible aggregation of the silica nanoparticles was prevented by precoating them with a neutral hydrophilic polymer. Both the elastic plateau modulus and the relaxation time increase slowly as the gel ages, indicating an increase both in the number of active bridges and in the strength with which the end blocks are adsorbed. The rate of this aging process can be increased significantly by applying a small shear stress to the sample. Our results indicate that charge-driven bridging of nanoparticles by triblock copolymers is a promising strategy for thickening of aqueous particle containing materials, such as water-based coatings. 相似文献
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In a twisted nematic layer oppositely twisted regions can exist, separated by a disclination line, as demonstrated by the orientation of Williams domains. The disclination line can move under the influence of a magnetic field. Measurements of the velocity as a function of magnetic field strength and sample thickness lead to an estimate of the size of the disclination core. 相似文献
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