Separation and Determination of Chloroplast Pigments from Spinach by Thin-Layer Chromatography: a Student Laboratory Experiment

JPC – Journal of Planar Chromatography – Modern TLC -     

Recent advances in the thin-layer chromatography of pesticides: a review

Advances in the applications of thin-layer chromatography (TLC) and high-performance TLC for the separation, detection, identification, and determination of pesticides, other agrochemicals, and related compounds are reviewed for the period 2000-2002. Analyses are described for a variety of samples, such as food, biological, and environmental samples, and for residues of pesticides of various types, including insecticides, herbicides, and fungicides, belonging to different chemical classes. References are included for residue analysis, hydrophobicity studies, and the use of TLC and thin-layer radiochromatography for studies of pesticide metabolism, degradation, uptake, and related topics.     

Determination of chlorophenoxy acid herbicides by densitometry on thin layer chromatograms

A quantitative in situ t.l.c. method for the determination of chlorophenoxy acid herbicides and their salts in waters is described. The detection limit (1 ppb) is similar to that obtainable by gas chromatography, but no derivatization is needed. Silica gel G plates are pre-impregnated with a sensitized silver nitrate reagent, and the spots are scanned with a densitometer after u.v. irradiation. Linear calibration graphs were obtained in the range 100–1000 ng for most of the herbicides studied. Preliminary cleanup methods are discussed.     

High-Performance Thin-Layer Chromatographic Analysis of the Polar Lipid Content of Human Urine and Urine from BALB/c Mice Experimentally Infected with Echinostoma caproni

JPC – Journal of Planar Chromatography – Modern TLC - High-performance thin-layer chromatography was used to examine the phospholipid profiles in the urine of Echinostoma caproni...     

A review of advances in two-dimensional thin-layer chromatography

Although there are many simple thin-layer chromatography (TLC) separations, many more are complex and involve more than a few components, that means having to use special high-performance TLC (HPTLC) plates or microspotting or banding devices to increase its resolving power if developing in only one direction. However, adding a second development to perform two-dimensional TLC (2D TLC) allows even better resolution of complex samples. This is because different modes of chromatography are being invoked by the use of one stationary phase with two mobile phases, bilayer plates, graft TLC, or multidimensional TLC. This paper is a review of recent applications that have benefitted from using 2D TLC in its various forms. They were chosen for their variety of sample types as well as the unique choices of plates and/or mobile phases made by the researchers to yield improved separations.