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Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dots 总被引:1,自引:0,他引:1
Roya Zekavati Shahabeddin Safi Seyed Jamal Hashemi Tavoos Rahmani-Cherati Meisam Tabatabaei Afshin Mohsenifar Mansour Bayat 《Mikrochimica acta》2013,180(13-14):1217-1223
We report on a competitive immunoassay for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET) from anti-aflatoxin B1 antibody (immobilized on the shell of CdTe quantum dots) to Rhodamine 123 (Rho 123-labeled aflatoxin B1 bound to albumin). The highly specific immunoreaction between the antibody against aflatoxin B1 on the QDs and the labeled-aflatoxin B1 brings the Rho 123 fluorophore (acting as the acceptor) and the QDs (acting as the donor) in close spatial proximity and causes FRET to occur upon photoexcitation of the QDs. In the absence of unlabeled aflatoxin B1, the antigen-antibody complex is stable, and strong emission resulting from the FRET from QDs to labeled aflatoxin B1 is observed. In the presence of aflatoxin B1, it will compete with the labeled aflatoxin B1-albumin complex for binding to the antibody-QDs conjugate so that FRET will be increasingly suppressed. The reduction in the fluorescence intensity of the acceptor correlates well with the concentration of aflatoxin B1. The feasibility of the method was established by the detection of aflatoxin B1 in spiked human serum. There is a linear relationship between the increased fluorescence intensity of Rho 123 with increasing concentration of aflatoxin B1 in spike human serum, over the range of 0.1–0.6 μmol·mL?1. The limit of detection is 2?×?10?11 M. This homogeneous competitive detection scheme is simple, rapid and efficient, and does not require excessive washing and separation steps. Figure
A nanobiosensor has been fabricated based on a competitive immunoassay for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET). In the presence of aflatoxin B1, it will compete with the labeled aflatoxin B1-albumin complex for binding to the antibody-QDs conjugate so that FRET will be increasingly suppressed. 相似文献
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Faramarz Rostami Charati Zinatossadat Hossaini Mohammad R. Hosseini‐Tabatabaei 《Journal of heterocyclic chemistry》2012,49(1):154-160
A straightforward and efficient method for the synthesis of 1,2‐dihydroisoquinolines via a one‐pot, three‐component reaction of isoquinoline, activated acetylenes, and NH‐acids in water at 70°C without using any catalyst is reported. The method offers several advantages including high yields of products and an easy workup procedure. J. Heterocyclic Chem., (2012). 相似文献
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Four-point bend specimen is one of the most important specimens of the fracture mechanics because it can produce mixed modes I and II. Therefore, computation of stress intensity factors in this specimen is of practical interest. Several relations have been suggested that no one of them has completely considered the effects of the loading point and crack geometry. In this paper, mixed mode stress intensity factors of the bend specimen are computed by finite element method (FEM) and after validating by comparing with the available results in the literature, the results will be assessed to determine the effects of different crack location and loading distances from the middle of the specimen. Finally, two new coefficients comprising these effects are introduced. 相似文献
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Eshtiagh-Hosseini H Housaindokht MR Beyramabadi SA Tabatabaei SH Esmaeili AA Khoshkholgh MJ 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,78(3):1046-1050
A new N,N'-dipyridoxyl(1,4-butanediamine) [=H(2)BS] Schiff-base ligand and its Cu(II) salen complex, [Cu(BS)(H(2)O)(CH(3)OH)], were synthesized and characterized by IR, UV-vis, (1)H NMR, mass spectrometry and elemental analysis. Also, full optimization of the geometries, (1)H NMR chemical shifts (for the H(2)BS) and vibrational frequencies were calculated by using density functional theory (DFT) method. Structure of the H(2)BS ligand is not planar, i.e. two pyridine rings are not in the same plane. In the structure of the Cu complex, the Schiff-base ligand acts as a dianionic tetradentate ligand in N, N, O(-), O(-) manner. The coordinating atoms of BS(2-) occupy equatorial positions of the octahedral complex, where the H(2)O and CH(3)OH ligands locate at axial positions. The calculated results are in good agreement with the experimental data, confirming the suitability of the proposed and optimized structures for the H(2)BS ligand and its Cu complex. 相似文献
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Kazem Bitaghsir Fadafan Seyed Kamal Tabatabaei 《The European Physical Journal C - Particles and Fields》2014,74(4):1-11
Nuclear effects in deep inelastic scattering at low $x$ are phenomenologically described changing the typical dynamical and/or kinematical scales characterizing the free nucleon case. In a holographic approach, this rescaling is an analytical property of the computed structure function $F_2(x,Q^2)$ . This function is given by the sum of a conformal term and of a contribution due to quark confinement, depending on IR hard-wall parameter $z_0$ and on the mean square distances, related to a parameter $Q^\prime $ , among quarks and gluons in the target. The holographic structure function per nucleon in a nucleus $A$ is evaluated showing that a rescaling of the typical nucleon size, $z_0$ and $Q^\prime $ , due to nuclear binding, can be reabsorbed in a $Q^2$ -rescaling scheme. The difference between neutron and proton structure functions and the effects of the longitudinal structure functions can also be taken into account. The obtained theoretical results favorably compare with the experimental data. 相似文献
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Mehrpooya Mehdi Tabatabaei Seyyed Hessamoddin Pourfayaz Fathollah Ghorbani Bahram 《Journal of Thermal Analysis and Calorimetry》2021,145(5):2547-2569
Journal of Thermal Analysis and Calorimetry - Solar thermochemical reactors have been considered in recent studies because of converting the solar energy to a fuel, which is called solar fuel. In... 相似文献
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Pt(II) complexes immobilized on polymer‐modified magnetic carbon nanotubes as a new platinum drug delivery system 下载免费PDF全文
Seyed Jamal Tabatabaei Rezaei Ali Hesami Hossein Khorramabadi Vahid Amani Asemeh Mashhadi Malekzadeh Ali Ramazani Hassan Niknejad 《应用有机金属化学》2018,32(7)
We combine nanotechnology and chemical synthesis to create a novel multifunctional platinum drug delivery vehicle based on magnetic carbon nanotubes (multiwall carbon nanotubes/Fe3O4@poly(citric acid)/cis‐[(Pt(1,7‐phenanthroline)(DMSO)Cl2)]‐b‐poly(ethylene glycol) (MCNTs/FO@PC/Pt(II)‐b‐PEG)) for targeted cancer therapy. MCNTs/FO@PC/Pt(II)‐b‐PEG was conveniently prepared by conjugating cis‐[Pt(1,7‐phenanthroline)(DMSO)Cl2] complex to MCNTs/FO@PC‐b‐PEG via strong hydrogen‐bonding interactions. In comparison with free cisplatin and Pt(II) complex, MCNTs/FO@PC/Pt(II)‐b‐PEG shows higher solubility in aqueous solution and higher cytotoxicity towards human cervical cancer HeLa cells and human breast cancer MDA‐MB‐231 cells. In vitro release experiments revealed that the platinum drug‐loaded delivery system is relatively stable under physiological conditions (pH = 7.4 and 37 °C) but susceptible to acidic environments (pH = 5.6 and 37 °C) which would trigger the release of loaded drugs. Fluorescence microscopy studies revealed that this magnetic nanohybrid system possesses marked cell‐specific targeting in vitro in the presence of an external magnetic field. The results indicated that the prepared superparamagnetic MCNTs/FO@PC/Pt(II)‐b‐PEG nanohybrid system is a promising candidate for inhibiting the proliferation of cancer cells. 相似文献