Die Methoden zur direkten Messung des osmotischen Drucks

Ohne Zusammenfassung     

Signal modelization for improved precision of assessment of minimum and mean telomere lengths

Telomere length is an important measure of cell and tissue regenerative capacities. The mean telomere length is classically used as global indicator of a tissue telomere length. In skeletal muscle, which is made of postmitotic myonuclei and satellite cells (muscle stem cells), minimum telomere length is also used to assess the telomere length of satellite cells and newly incorporated myonuclei. At present, the estimation of the method reproducibility during the assessment of mean and minimum telomere length using Southern blot analysis has never been documented. The aim of this report is to describe a signal modelization for improved precision of assessment of minimum and mean telomere lengths and to document the method reproducibility. Telomeres are assessed using a Southern technique where the gel is directly hybridized with the specific probe without the membrane-transferring step in order to prevent telomeric low signal loss. We found that the improved signal analysis for determination of telomere length is associated with coefficients of variation ranging from 1.37 to 4.29% for the mean telomeric restriction fragment (TRF) length and from 2.04 to 4.95% for the minimum TRF length. Improved method reproducibility would allow saving time and biological material as duplicate and triplicate measurement of the same sample is no longer required.     

Clebsch–Gordan and Racah–Wigner Coefficients for a Continuous Series of Representations of ?q (??(2, ℝ))

The decomposition of tensor products of representations into irreducibles is studied for a continuous family of integrable operator representations of U _q (sl(2,ℝ). It is described by an explicit integral transformation involving a distributional kernel that can be seen as an analogue of the Clebsch–Gordan coefficients. Moreover, we also study the relation between two canonical decompositions of triple tensor products into irreducibles. It can be represented by an integral transformation with a kernel that generalizes the Racah–Wigner coefficients. This kernel is explicitly calculated. Received: 9 August 2000 / Accepted: 2 July 2001