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将巯基修饰的核酸适配子(aptmer)偶联到金纳米粒子(AuNPs)表面,制备出朊蛋白特异性的Apt-AuNPs纳米光学探针,并成功应用到细胞表面朊蛋白的光散射成像和电子透射显微成像分析.通过对Apt-AuNPs探针进入细胞的途径及其在细胞内命运的进一步研究表明,窖蛋白介导的内吞作用可能是其进入细胞的一个重要途径.Apt-AuNPs纳米探针制备简单、成本低廉,可能被广泛应用于生物医学成像领域. 相似文献
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Zhang Kai Jiang PingPing Nie Zhixin Gu Qian Zhang Pingbo 《Journal of Solid State Electrochemistry》2021,25(6):1825-1834
Journal of Solid State Electrochemistry - The development of transition metal-based catalysts for hydrogen evolution reaction with high active and stable performance is becoming an important means... 相似文献
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朊病毒病是一类累及多种动物和人类中枢神经系统退行性疾病,但至今针对这类疾病尚无有效的治疗方法.考虑到在180位的缬氨酸突变为异亮氨酸的180I突变蛋白的突变位点与朊蛋白181位的糖基化位点非常接近,其生物化学性质对朊病毒病的影响非常重要.本文针对180I突变蛋白的182-190段序列设计了KNFTK、KTDVE、EMMKE和EVVKK等四种αxyzβ型多肽.研究发现,四种多肽中只有EVVKK能稳定蛋白的构象,同时诱导β-折叠向α-螺旋的转变,而其他三种蛋白对180I的结构基本没有影响.该结论对于开发多肽药物并进一步用于临床治疗具有一定的借鉴作用. 相似文献
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HU PingPing PENG Li ZHEN ShuJun CHEN LiQiang XIAO SaiJin & HUANG ChengZhi Key Laboratory on Luminescence Real-Time Analysis Ministry of Education College of Chemistry Chemical Engineering Southwest University Chongqing China College of Life Science College of Pharmaceutical Sciences 《中国科学:化学》2010,(4)
Homochirality in life has always been a driving force in scientific research and natural exploration. It has not been satisfactorily explained, and systematic investigations are necessary. This paper reported a homochiral expression of proteins dependent on the stirring direction of growing media. By controlling the stirring direction clockwise (CW) and anticlockwise (ACW) of the culture medium, proteins with distinct secondary structures were obtained, and D-amino acid may be included in the protein cultur... 相似文献
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An ionic hybrid catalyst 1,1’-(butane-1,4-diyl)-bis(3-methylimidazolium) phosphotungstate(abbreviated [Dmim] 1.5 PW) has been prepared by anion-exchange of the divalent ionic liquid(IL) 1,1’-(butane-1,4-diyl)-bis(3-methylimidazolium) di(bromide) with the Keggin phosphotungstic acid H 3 PW 12 O 40,and characterized by IR,1 H NMR,13 C NMR,ESI-MS,TG,SEM,XRD,BET surface area measurements,elemental analysis,and melting point.The hybrid material was evaluated as a catalyst for the oxidation of alcohols with aqueous hydrogen peroxide under various conditions.The catalytic performance of [Dmim] 1.5 PW was also compared with related catalysts bearing other cations or anions.The new hybrid [Dmim] 1.5 PW proved to be an efficient liquid-solid heterogeneous catalyst for H2O2-based oxidation of alcohols,with the advantages of high conversion and selectivity,easy recovery,and quite good reusability. 相似文献
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经典的用回归模型进行统计控制中的问题 总被引:1,自引:0,他引:1
利用回归模型进行统计控制,在实际工作中一直得到广泛应用。通常采用的方法是利用回归模型y^=a+bx进行逆估计,即根据回归模型的变换x=(y^-a)/b,由应变量Y的取值范围反推自变量X的取值范围。本文指出这种方法是很不合理的;进一步提出二种相对合理取代的统计方法。 相似文献
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PingPing Hu Li Peng ShuJun Zhen LiQiang Chen SaiJin Xiao ChengZhi Huang 《中国科学:化学(英文版)》2010,53(4):792-796
Homochirality in life has always been a driving force in scientific research and natural exploration. It has not been satisfactorily explained, and systematic investigations are necessary. This paper reported a homochiral expression of proteins dependent on the stirring direction of growing media. By controlling the stirring direction clockwise (CW) and anticlockwise (ACW) of the culture medium, proteins with distinct secondary structures were obtained, and D-amino acid may be included in the protein cultured with the stirring direction of ACW. Considering the effect of force fields, which might affect the process of folding and refolding of cellular protein in this report, the control of force fields might be a good way to prepare asymmetric drugs, and the rotational direction of the earth is possibly related to the chirality in primitive life molecules. 相似文献
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LiQiang Chen PingPing Hu Li Zhang SiZhou Huang LingFei Luo ChengZhi Huang 《中国科学:化学(英文版)》2012,55(10):2209-2216
Graphene possesses unique physical and chemical properties, which have inspired a wide range of potential biomedical applications. However, little is known about the adverse effects of graphene on the human body and ecological environment. The purpose of our work is to make assessment on the toxicity of graphene oxide (GO) against human cell line (human bone marrow neuroblastoma cell line and human epithelial carcinoma cell line) and zebrafish (Danio rerio) by comparing the toxic effects of GO with its sister, multi-walled carbon nanotubes (MWNTs). The results show that GO has a moderate toxicity to organisms since it can induce minor (about 20%) cell growth inhibition and slight hatching delay of zebrafish embryos at a dosage of 50 mg/L, but did not result in significant increase of apoptosis in embryo, while MWNTs exhibit acute toxicity leading to a strong inhibition of cell proliferation and serious morphological defects in developing embryos even at relatively low concentration of 25 mg/L. The distinctive toxicity of GO and MWNTs should be ascribed to the different models of interaction between nanomaterials and organisms, which arises from the different geometric structures of nanomaterials. Collectively, our work suggests that GO does actual toxicity to organisms posing potential environmental risks and the result is also shedding light on the geometrical structure-dependent toxicity of graphitic nanomaterials. 相似文献
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SUN PingPing LIU DeAn ZHANG YanLi LI XiaoYan ZHANG Yan & ZHU JianQiang National Laboratory on High Power Laser Physics 《中国科学:物理学 力学 天文学(英文版)》2011,(3)
The theoretical model of spatial noise passing through a spatial filter is established in high power laser system under the small signal approximation. The transmission characteristic for a noise signal passing through spatial filters with different magnifications is analyzed by numerical simulation, according to the actual structure of the high power laser system. The results show that the spatial modulation period of low-frequency noise getting through the pinhole will be proportional to the magnification... 相似文献