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1.
Mohsen Keyvanfard Nazila Najjarian Khadijeh Alizad 《Journal of Analytical Chemistry》2017,72(10):1045-1050
In this study, we describe the application of carbon paste electrode modified with multiwall carbon nanotubes as a voltammetric sensor for determination of penicillamine (PA) in the presence of chlorpromazine as a mediator. This modified electrode showed very efficient electrocatalytic activity for the anodic oxidation of PA. The peak current of linear sweep voltammograms of PA increased linearly with it’s concentration in the range of 0.5–500 μM PA. The detection limit for PA was 0.2 μM. The RSDs for 1.0 and 10.0 μM PA were 1.1 and 1.7%, respectively. The proposed sensor was successfully applied for the determination of PA in human urine and tablet. 相似文献
2.
Noureddine Bencheikh Mohamed Bouhrim Loubna Kharchoufa Omkulthom Mohamed Al Kamaly Hamza Mechchate Imane Es-safi Ahmed Dahmani Sabir Ouahhoud Soufiane El Assri Bruno Eto Mohamed Bnouham Mohammed Choukri Mostafa Elachouri 《Molecules (Basel, Switzerland)》2021,26(16)
Zizyphus lotus L. (Desf.) (Z. lotus) is a medicinal plant largely distributed all over the Mediterranean basin and is traditionally used by Moroccan people to treat many illnesses, including kidney failure. The nephrotoxicity of gentamicin (GM) has been well documented in humans and animals, although the preventive strategies against it remain to be studied. In this investigation, we explore whether the extract of Zizyphus lotus L. (Desf.) Fruit (ZLF) exhibits a protective effect against renal damage produced by GM. Indeed, twenty-four Wistar rats were separated into four equal groups of six each (♂/♀ = 1). The control group was treated orally with distilled water (10 mL/kg); the GM treated group received distilled water (10 mL/kg) and an intraperitoneal injection of GM (80 mg/kg) 3 h after; and the treated groups received ZLF extract orally at the doses 200 or 400 mg/kg and injected intraperitoneally with the GM. All treatments were given daily for 14 days. At the end of the experiment, the biochemical parameters and the histological observation related the kidney function was explored. ZLF treatment has significantly attenuated the nephrotoxicity induced by the GM. This effect was indicated by its capacity to decrease significantly the serum creatinine, uric acid, urea, alkaline phosphatase, gamma-glutamyl-transpeptidase, albumin, calcium, sodium amounts, water intake, urinary volume, and relative kidney weight. In addition, this effect was also shown by the increase in the creatinine clearance, urinary creatinine, uric acid, and urea levels, weight gain, compared to the rats treated only with the GM. The hemostasis of oxidants/antioxidants has been significantly improved with the treatment of ZLF extract, which was shown by a significant reduction in malondialdehydes levels. Histopathological analysis of renal tissue was correlated with biochemical observation. Chemical analysis by HPLC-DAD showed that the aqueous extract of ZLF is rich in phenolic compounds such as 3-hydroxycinnamic acid, catechin, ferulic acid, gallic acid, hydroxytyrosol, naringenin, p- coumaric Acid, quercetin, rutin, and vanillic acid. In conclusion, ZLF extract improved the nephrotoxicity induced by GM, through the improvement of the biochemical and histological parameters and thus validates its ethnomedicinal use. 相似文献
3.
Oliver M Ahmad A Kamaly N Perouzel E Caussin A Keller M Herlihy A Bell J Miller AD Jorgensen MR 《Organic & biomolecular chemistry》2006,4(18):3489-3497
Cellular entry of imaging probes, such as contrast agents for magnetic resonance imaging (MRI), is a key requirement for many molecular imaging studies, particularly imaging intracellular events and cell tracking. Here, we describe the successful development and in vitro analysis of MAGfect, a novel liposome formulation containing a lipidic gadolinium contrast agent for MRI, Gd-DOTA-Chol , designed to enter and label cells. Liposome formulation and cell incubation time were optimised for maximum cellular uptake of the imaging probe in a variety of cell lines. MRI analysis of cells incubated with MAGfect showed them to be highly MRI active. This formulation was examined further for cytotoxicity, cell viability and mechanism of cell labelling. One of the key advantages of using MAGfect as a labelling vehicle arises from its potential for additional functions, such as concomitant drug or gene delivery and fluorescent labelling. The gadolinium liposome was found to be an effective vehicle for transport of plasmid DNA (pDNA) into cells and expression levels were comparable to the commercial transfection agent Trojene. 相似文献
4.
Palladium(II) Immobilized Onto the Glucose Functionalized Magnetic Nanoparticle as a New and Efficient Catalyst for the One‐pot Synthesis of Benzoxazoles 下载免费PDF全文
Palladium(II) have been immobilized into the nano magnetic Fe3O4 which was functionalized with glucose in order to achieve a one‐pot synthesis of 2‐substituted benzoxazole derivatives with high yields in the diverse range of organic solvents. The nano catalyst is highly dispersive in polar solvents and can be easily recovered and reused for 6 runs without significant loss of its activity. Finally, the catalyst was fully characterized by FT‐IR, TGA, CHN, SEM, EDX and atomic absorption spectroscopy. 相似文献
5.
Dr. Jun Wu Dr. Nazila Kamaly Dr. Jinjun Shi Lili Zhao Dr. Zeyu Xiao Geoffrey Hollett Rohit John Shaunak Ray Dr. Xiaoyang Xu Dr. Xueqing Zhang Prof. Philip W. Kantoff Prof. Omid C. Farokhzad 《Angewandte Chemie (International ed. in English)》2014,53(34):8975-8979
One limitation of current biodegradable polymeric nanoparticles is their inability to effectively encapsulate and sustainably release proteins while maintaining protein bioactivity. Here we report the engineering of PLGA–polycation nanoparticles with a core–shell structure that act as a robust vector for the encapsulation and delivery of proteins and peptides. The optimized nanoparticles can load high amounts of proteins (>20 % of nanoparticles by weight) in aqueous solution without organic solvents through electrostatic interactions by simple mixing, thereby forming nanospheres in seconds with diameters <200 nm. The relationship between nanosphere size, surface charge, PLGA–polycation composition, and protein loading is also investigated. The stable nanosphere complexes contain multiple PLGA–polycation nanoparticles, surrounded by large amounts of protein. This study highlights a novel strategy for the delivery of proteins and other relevant molecules. 相似文献
6.
Imane Es-safi Hamza Mechchate Amal Amaghnouje Omkulthom Mohamed Al Kamaly Fatima Zahra Jawhari Hamada Imtara Andriy Grafov Dalila Bousta 《Molecules (Basel, Switzerland)》2021,26(7)
Depression and anxiety are major mental health problems in all parts of the world. These illnesses are associated with a number of risk factors, including oxidative stress. Psychotropic drugs of a chemical nature have demonstrated several side effects that elevated the impact of those illnesses. Faced with this situation, natural products appear to be a promising alternative. The aim of this study was to evaluate the anxiolytic and antidepressant effects of the Petroselinum sativum polyphenols in vivo, as well as its correlated antioxidant properties in vitro. Anxiolytic activity of the extract (50 and 100 mg/kg) was evaluated using the open field and the light-dark chamber tests, while the antidepressant activity was evaluated using the forced swimming test. The antioxidant activity of the extract was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical test and the FRAP (iron-reducing capacity) test. The phenolic extract showed very powerful anxiolytic and antidepressant-like effects, especially at a dose of 100 mg/kg, decreasing the depressive behavior in mice (decreased immobility time) and also the anxiolytic behavior (tendency for discovery in the center and illuminated areas) better even than those of paroxetine and bromazepam (classic drugs) concomitant with those results the extract also showed an important antioxidant capacity. These preliminary results suggest that Petroselinum sativum exhibits anxiolytic and antidepressant potential for use as a complement or independent phytomedicine to treat depression and anxiety. 相似文献
7.
Afshin Derakhshani Zeinab Rostami Hossein Safarpour Mahdi Abdoli Shadbad Niloufar Sadat Nourbakhsh Antonella Argentiero Sina Taefehshokr Neda Jalili Tabrizi Omid Kooshkaki Reza Vaezi Astamal Pankaj Kumar Singh Nima Taefehshokr Nazila Alizadeh Nicola Silvestris Behzad Baradaran 《Molecules (Basel, Switzerland)》2021,26(8)
Over the past decade, there have been remarkable advances in understanding the signaling pathways involved in cancer development. It is well-established that cancer is caused by the dysregulation of cellular pathways involved in proliferation, cell cycle, apoptosis, cell metabolism, migration, cell polarity, and differentiation. Besides, growing evidence indicates that extracellular matrix signaling, cell surface proteoglycans, and angiogenesis can contribute to cancer development. Given the genetic instability and vast intra-tumoral heterogeneity revealed by the single-cell sequencing of tumoral cells, the current approaches cannot eliminate the mutating cancer cells. Besides, the polyclonal expansion of tumor-infiltrated lymphocytes in response to tumoral neoantigens cannot elicit anti-tumoral immune responses due to the immunosuppressive tumor microenvironment. Nevertheless, the data from the single-cell sequencing of immune cells can provide valuable insights regarding the expression of inhibitory immune checkpoints/related signaling factors in immune cells, which can be used to select immune checkpoint inhibitors and adjust their dosage. Indeed, the integration of the data obtained from the single-cell sequencing of immune cells with immune checkpoint inhibitors can increase the response rate of immune checkpoint inhibitors, decrease the immune-related adverse events, and facilitate tumoral cell elimination. This study aims to review key pathways involved in tumor development and shed light on single-cell sequencing. It also intends to address the shortcomings of immune checkpoint inhibitors, i.e., their varied response rates among cancer patients and increased risk of autoimmunity development, via applying the data from the single-cell sequencing of immune cells. 相似文献
8.
Anjan Biswas Ghodrat Ebadi Houria Triki Ahmet Yildirim Nazila Yousefzadeh 《Results in Mathematics》2013,63(1-2):687-703
This paper studies the KdV–Caudrey–Dodd–Gibbon equation. The modified F-expansion method, exp-function method as well as the G′/G method are used to extract a few exact solutions to this equation. Later, the ansatz method is used to obtain the topological 1-soliton solution to this equation. The constraint conditions are also obtained that must remain valid for the existence of these solutions. 相似文献
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10.
Firouz Matloubi Moghaddam Behzad Koushki Foroushani Maryam Sobhani Nazila masoud Mohammad Reza Khodabakhshi Ng Seik Weng 《Tetrahedron》2013
Indoline-2-thiones (5) were applied as 1,3-dinucleophiles in a tandem reaction with Baylis–Hillman adduct acetates (4) to give novel tetrahydrothiopyrano[2,3-b]indole skeletons (6). The effect of different solvents, bases, and catalysts on the yields and stereochemical outcome was studied in detail. The results indicated that acetonitrile as solvent and K2CO3 as base, under reflux conditions, were the optimum conditions. Products 6a–6l were obtained in high diastereoselectivity and yield (up to 94%). 相似文献