Stability-Indicating HPTLC Method for Simultaneous Determination of Ezetimibe and Simvastatin

Simvastatin and ezetimibe are used to treat hyperlipidemia. A simple, selective and stability-indicating HPTLC method has been established for analysis of simvastatin and ezetimibe. The method has been validated so that both drugs can routinely be analyzed simultaneously. The method uses aluminum-backed silica gel 60F₂₅₄ TLC plates as stationary phase with n-hexane–acetone 6:4 (v/v) as mobile phase. Densitometric analysis of both drugs was carried out in absorbance mode at 234 nm. This system was found to give compact bands for simvastatin and ezetimibe (R _F 0.39 ± 0.05 and 0.50 ± 0.05, respectively). Linear relationships were obtained between response and amount of drug in the range 200–1,600 ng per band with high correlation coefficients (r ² = 0.9917 ± 0.0018 for simvastatin and r ² = 0.9927 ± 0.0021 for ezetimibe). The method was validated for precision, robustness, and recovery. The limits of detection and quantitation were 25 and 150 ng per band, respectively. Simvastatin and ezetimibe were subjected degradation by acid, pH 6.8 phosphate buffer, oxidation, dry heat, and wet heat. The degradation products were well resolved from the pure drug with significantly different R _F values. Because the method could effectively separate the drug from its degradation products, it can be used for stability-indicating analysis.     

Quantitative Analysis of Flavonoids in Annona squamosa Leaf Extracts and Its Pellet Formulation by Validated High-Performance Thin-Layer Chromatographic Technique

JPC – Journal of Planar Chromatography – Modern TLC - Annona squamosa, commonly known as custard apple, possesses various medicinal properties such as antimicrobial, insecticidal,...     

On a test of equality of two-parameter exponential distributions

In this paper, an asymptotic expansion of the distribution of the statistic for testing the equality of p two-parameter exponential distributions is obtained upto the order n^?4 with the second term of the order n^?3 where n is the size of the sample drawn from the i th exponential population. The asymptotic expansion can therefore be used to obtain accurate approximations to the critical values of the test statistic even for comparatively small values of n. Also we have shown that F-tables can be used to test the hypothesis when the sample size is moderately large.     

HPTLC Determination of Cefpodoxime Proxetil in Formulations

An HPTLC method has been developed that coelutes both the isomers of cefpodoxime proxetil (CFP). CFP was chromatographed on a silica gel 60 F₂₅₄ TLC plate using toluene:acetonitrile (6:4) as a mobile phase and was quantified at 234 nm. The method was validated with respect to linearity, accuracy, precision and specificity. The limit of detection and limit of quantification for CFP were found to be 0.150 and 0.4 μg spot⁻¹, respectively. The proposed method was successfully used to determine the amount of CFP present in the marketed tablets and self-nanoemulsifying systems.

     

Nonnull distributions of some statistics associated with testing for the equality of two covariance matrices

The nonnull distribution of some statistics, used for testing Σ₁ = Σ₂ are obtained as mixtures of incomplete beta functions as well as mixtures of incomplete gamma functions. The introduction of the convergence factors and certain recurrence relations are useful in the computation of the power of the tests as well as computation of exact percentage points for tests of significance.     

HPTLC Determination of Cefpodoxime Proxetil in Formulations

An HPTLC method has been developed that coelutes both the isomers of cefpodoxime proxetil (CFP). CFP was chromatographed on a silica gel 60 F₂₅₄ TLC plate using toluene:acetonitrile (6:4) as a mobile phase and was quantified at 234 nm. The method was validated with respect to linearity, accuracy, precision and specificity. The limit of detection and limit of quantification for CFP were found to be 0.150 and 0.4 μg spot^?1, respectively. The proposed method was successfully used to determine the amount of CFP present in the marketed tablets and self-nanoemulsifying systems.     

The distribution of the sphericity test criterion

The exact distribution of Mauchly's sphericity test criterion W = |S|/[trS/p]^p, when S is the sum of product matrix from a sample of size N taken from a p-variate normal population, is obtained using contour integration and methods similar to those of Nair and Box. Tables of percentage points for p = 4(1)10, α = 0.01 and 0.05, and various values of N (including small) are given and comparisons made with approximate percentage points using methods of Box, Mauchly, Tukey and Wilks, and Davis.     

Asymptotic distributions of the likelihood ratio test statistics for covariance structures of the complex multivariate normal distributions

In this paper, the authors derived asymptotic expressions for the null distributions of the likelihood ratio test statistics for multiple independence and multiple homogeneity of the covariance matrices when the underlying distributions are complex multivariate normal. Also, asymptotic expressions are obtained in the non-null cases for the likelihood ratio test statistics for independence of two sets of variables and the equality of two covariance matrices. The expressions obtained in this paper are in terms of beta series. In the null cases, the accuracy of the first terms alone is sufficient for many practical purposes.     

Distribution of the likelihood ratio criterion for testing Σ = Σ0, μ = μ0

The exact null distribution of the likelihood ratio criterion for testing H₀: Σ = Σ₀ and μ = μ₀ against alternatives H₁: Σ ≠ Σ₀ or μ ≠ μ₀ in N_p(μ, Σ) has been obtained as (a) a chi-square series and (b) a beta series. Percentage points have been tabulated for p = 2(1) 6, α = .005, .01, .025, .05, .1, and .25 and various values of sample size N.