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Purification and Structure Identification of Hyaluronic Acid 总被引:1,自引:0,他引:1
Shui Sheng HONG Jia CHEN Jian Guo ZHANG Yan Chun TAO Lan YingLIUCollege of Life Science Jilin University Changchun State Key Laboratory of Electroanalytical Chemistry Chinese Academy of Sciences ChangchunInstitute of Applied Chemistry Changchun Key Laboratory of Supermolecular Structure Spectroscopy of Educational Ministry Jilin University Changchun 《中国化学快报》2004,15(7):811-812
Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis,13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method. 相似文献
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