排序方式: 共有3条查询结果,搜索用时 109 毫秒
1
1.
Charlton C Gubala V Gandhiraman RP Wiechecki J Le NC Coyle C Daniels S Maccraith BD Williams DE 《Journal of colloid and interface science》2011,354(1):405-409
We report a method for studying nanoparticle-biosensor surface interactions based on total internal reflection fluorescence (TIRF) microscopy. We demonstrate that this simple technique allows for high throughput screening of non-specific adsorption (NSA) of nanoparticles on surfaces of different chemical composition. Binding events between fluorescent nanoparticles and functionalized Zeonor? surfaces are observed in real-time, giving a measure of the attractive or repulsive properties of the surface and the kinetics of the interaction. Three types of coatings have been studied: one containing a polymerized aminosilane network with terminal -NH(2) groups, a second film with a high density of -COOH surface groups and the third with sterically restraining branched poly(ethylene)glycol (PEG) functionality. TIRF microscopy revealed that the NSA of nanoparticles with negative surface charge on such modified coatings decreased in the following order -NH(2)>-branched PEG>-COOH. The surface specificity of the technique also allows discrimination of the degree of NSA of the same surface at different pH. 相似文献
2.
Optical Sol-Gel-Based Dissolved Oxygen Sensor: Progress Towards a Commercial Instrument 总被引:1,自引:0,他引:1
Mcdonagh C.M. Shields A.M. Mcevoy A.K. Maccraith B.D. Gouin J.F. 《Journal of Sol-Gel Science and Technology》1998,13(1-3):207-211
A dissolved oxygen sensor based on fluorescence quenching of the oxygen-sensitive ruthenium complex, [Ru(II)-tris(4,7-diphenyl-1,10-phenanthroline]2+, which has been immobilized in a porous silica sol-gel-derived film, is reported. Ormosil sensing films were fabricated using modified silica precursors such as methyltriethoxysilane (MTEOS) and ethyltriethoxysilane (ETEOS) and were dip-coated onto planar glass substrates. Tailoring of the films for dissolved oxygen (DO) sensing is described whereby sensor response is optimized by maximizing film hydrophobicity by the use of the modified precursors. Sensor performance parameters such as limit of detection and sensor resolution are reported. Issues such as dye leaching and photobleaching are discussed. Progress towards a commercial instrument is reported. 相似文献
3.
P. Kiernan C. Mcdonagh B. D. Maccraith K. Mongey 《Journal of Sol-Gel Science and Technology》1994,2(1-3):513-517
Sol-gel silica thin films, produced by a dip-coating process, were impregnated with the complexes Ru(bpy)
3
2+
and Ru(Ph2phen)
3
2+
. For each complex ruthenium fluorescence was quenched in the presence of oxygen. Intensity and decay time Stern-Volmer plots were produced for both complexes. The optical decay times were analysed in terms of one quenched and one unquenched component, the latter arising from the fraction of complex molecules which are inaccessible to oxygen. All the data were consistent with the predominance of dynamic quenching in these systems. The feasibility of an oxygen sensor based on decay times was discussed. 相似文献
1