Capillary-channeled polymer fibers as stationary phases in liquid chromatography separations

A method utilizing capillary-channeled polymer (C-CP) fibers as stationary phases in high-performance liquid chromatographic separations has been investigated. Polymeric fibers of differing backbones (polypropylene and polyester) having nominal diameters of approximately 50 and approximately 35 microm and a channeled structure on their periphery were packed into stainless steel tubing (305 x 4.6 mm I.D.) for use in reversed-phase separations of various mixtures. The fibers have eight channels running continuously along the axis which exhibit very high surface activity. As such, solvent transport is affected through the channels through wicking action. Bundles of 1000-3000 fibers are loaded co-linearly into the tubing, providing flow channels extending the entire length of the columns. As a result, backing pressures are significantly lowered (approximately 50% reduction) in comparison to packed-sphere columns. In addition, the capital costs of the fiber material (< US$0.25 per column) are very attractive. Flow-rates of up to 5 ml/min can be used to achieve near baseline separation of related compounds in reasonable run times, indicating very fast mobile phase mass transfer (C-terms). The polymer stationary phases demonstrate high selectivity for a wide variety of analytes with gradient elution employed successfully in many instances. Specifically, separations of three polyaromatic hydrocarbons (benzo[a]pyrene, chrysene, pyrene), mixtures of both organic and inorganic lead compounds [chlorotriethyllead, chlorotriphenyllead, lead nitrate, lead(II) phthalocyanine], and a lipid standard of triglycerides were accomplished on the polymeric stationary phases. Other species of biological interest, including groups of aliphatic and aromatic amino acids have also been effectively separated. The reversed-phase nature of the fiber surfaces is supported through atomic force microscopy measurements using hydrophilic and hydrophobic functionalized polystyrene beads as the probe tips. Separations of the various analytes demonstrate the feasibility of utilizing C-CP fibers as stationary phases in reversed-phase LC. It is envisioned that columns of this nature would be particularly useful in prep-scale separations as well as for immobilization matrices for organic constituents in aqueous environments.     

Dipolar interactions and origin of spin ice in ising pyrochlore magnets

Recent experiments suggest that the Ising pyrochlore magnets Ho2Ti2O7 and Dy2Ti2O7 display qualitative properties of the nearest-neighbor "spin ice" model. We discuss the dipolar energy scale present in both these materials and discuss how spin-ice behavior can occur despite the presence of long-range dipolar interactions. We present results of numerical simulations and a mean field analysis of Ising pyrochlore systems. Based on our quantitative theory, we suggest that the spin-ice behavior in these systems is due to long-range dipolar interactions, and that the nearest-neighbor exchange in Dy2Ti2O7 is antiferromagnetic.     

Biomedical and clinical applications of automated single cell electrophoresis

The automated single cell electrophoresis microscope Parmoquant has been used for the discrimination of lymphocytes and the study of the interaction of substances with cells and synthetic particles. Electrophoretic histograms allowed the determination of changes in the proportion of lymphocyte populations after kidney transplantation, during dialysis treatment, open heart surgery and during pregnancy. Discrimination of leukemic cells on the basis of electrophoresis was used as an additional parameter in diagnosis. In a mouse tumor model, histogram determination enabled the in vivo effect of the tumor necrosis factor on immune cells to be evaluated. Cell electrophoresis was shown to be suitable to detect the influence of antibodies, lectins and bacteria on the cell surface. Protein adsorption was studied on synthetic particles using cell electrophoresis. This method was applied to investigate the phenomena of blood interaction with biomaterials used in artificial organs and to determine differences in the protein composition of serum or other body fluids connected with diseases.     

Exact scalar field cosmologies in a higher derivative theory

We obtain exact cosmological solutions of a higher derivative theory described by the Lagrangian L=R+2αR ² in the presence of interacting scalar field. The interacting scalar field potential required for a known evolution of the FRW universe in the framework of the theory is obtained using a technique different from the usual approach to solve the Einstein field equations. We follow here a technique to determine potential similar to that used by Ellis and Madsen in Einstein gravity. Some new and interesting potentials are noted in the presence of R ² term in the Einstein action for the known behaviours of the universe. These potentials in general do not obey the slow rollover approximation.     

Orbital Angular Momentum in Scalar Diquark Model and QED

We compare the orbital angular momentum of the ‘quark’ in the scalar diquark model as well as that of the electron in QED (to order α) obtained from the Jaffe–Manohar decomposition to that obtained from the Ji relation. We estimate the importance of the vector potential in the definition of orbital angular momentum.     

low-frequency dispersion of surface conducting particles as measured by means of electrorotation

Electrorotation of glutaraldehyde-fixed red blood cells (RBCs) has been investigated in the frequency range from 16 Hz to 30 MHz. Special emphasis has been given to the low-frequency range (16 Hz–2 kHz). In this region, a distinctly new type of co-field rotation peak was observed. Native RBCs also show low-frequency rotation, but much less pronounced. The low-frequency rotation is nonmonotonous with regard to conductivity. With increasing external conductivity, the low-frequency rotation speed reaches a maximum of around 3.5 mS/m and decreases again when the external conductivity is increased further. The low-frequency co-field rotation peak was observed between 30 Hz and 70 Hz except at very low conductivity almost independent of electrolyte concentration. Decreasing surface charge density by means of neuraminidase led to a reduction of the rotation speed in the low-frequency range. The position of the peak remained unaffected by changes of the surface charge density. These experimental results are not consistent with the typical Pauli–Schwan- and Maxwell–Wagner-dispersion. They rather suggest the presence of a concentration polarization mechanism responsible for the low-frequency electrorotation peak.     

On the geometric isomers of ethyl 2-nitro-3-ethoxyacrylate,ethyl 2-nitro-3-phenylaminoacrylate and ethyl 2-cyano-3-phenylaminoacrylate

The title compounds exist as mixtures of Z,E-isomers. The assignment of the proton signals of PNMR spectra is performed empirically and by application of lanthanide shift reagents.     

An efficient one-pot synthesis of coumarin-amino acid derivatives as potential anti-inflammatory and antioxidant agents

Abstract

A series of seven coumarinyl-amino acid ester conjugates have been synthesized and characterized by NMR (¹H and 13C) and mass spectra. Further, the compounds were investigated for their therapeutic applications such as anti-inflammatory and antioxidant activities. Among the synthesized compounds most of the analogs showed good efficiency compared with the standard.     

A Comparison of Heuristics for the Discrete Cost Multicommodity Network Optimization Problem

In this paper, approximate solutions algorithms for discrete cost multicommodity network optimization problems are presented and compared. Firstly, extensions of classical greedy heuristics, based on link-rerouting and flow-rerouting heuristics, are presented in details. Secondly, a new approximate solution algorithm, which basically consists of a heuristic implementation of the exact Benders-type cutting plane generation method, is proposed. All these algorithms are extensively compared on randomly generated graphs up to 50 nodes and 90 links. It clearly appears that this new Benders-type approach is very promising since it produces the best heuristic solutions.     

The multi-layered network design problem

We address the problem of designing a network built on several layers. This problem occurs in practical applications but has not been studied extensively from the point of view of global optimisation, since the problem of designing a single-layered network is complex. An example of an application is the design of a virtual network (Internet Protocol) built on a sparse optical transport network.