Interaction of bis-aryl functionalized molecules with nucleosides and nucleic acids

A series of novel molecules with a cyclen(1,4,7,10-tetraazacyclododecane) moiety appended on and bearing different aromatic fragments in the structures were synthesized and characterized.The binding activities of these compounds towards DNA were systematically studied by spectroscopic,viscometric and gel electrophoresis methods.The results suggest that the stacking interaction plays an important role in improving the DNA binding ability of the compounds.The binding modes of the compounds towards DNA are als...     

Synthesis,DNA binding and cleavage studies of the copper(Ⅱ) complexes of PNA-cyclen conjugates

Two dinucleotide PNA-cyclen copper(II) complexes with α-PNA (P1) and classical PNA (P2) backbones were synthesized and characterized.The interactions between title complexes and DNA were investigated under physiological conditions.Fluorescence studies indicate that the binding ability of complex P1 to CT-DNA is as twice as that of P2.DNA melting experiments were also carried out and the results show that ΔTm caused by P1 is higher than that caused by P2.Agarose gel electrophoresis experiments demonstrate th...     

Synthesis and Antimicrobial Properties of the Novel Fluonnated Bis-ammonium Salts with Two Primary Amine Groups

In order to resolve the increasing resistance phenomena of the Gram-negative bacteria against single chain quaternary ammonium salts (QAS), lysine with a pedant fluorinated bis-ammonium salts was synthesized and its antimicrobial properties were evaluated in this work. The novel fluorinated bis-ammonium salts shows similar activity with conventional single chain quaternary ammonium salts against Gram-positive bacteria but stronger activity against Gram-negative bacteria and yeast compared with the single chained counterpart.     

基于大环多胺的新型线型聚合物的合成及与DNA相互作用的研究

设计合成了一种新型线形大环聚合多胺（POGEC）,该聚合物可通过1,3-丙二醇二缩水甘油基醚和1,7-二（二乙氧磷酰基）-1,4,7,10-四氮杂环十二烷反应得到．琼脂糖凝胶电泳和电子扫描光谱（SEM）显示POGEC与DNA有很强的结合能力．当生理浓度的氯化钠溶液加入到已经形成的POGEC／DNA复合物中,DNA可被有效释放．通过荧光光谱测量了POGEC与DNA的结合能力及紧缩能力．圆二色光谱表明POGEC的强结合力并未改变DNA的构象．