Spectrophotometric determination of uranium with 5-(2′-carboxyphenyl)azo-8-quinolinol in the non-ionic micellar medium of Triton X-100

Triton X-100, a non-ionic surfactant, has been used to sensitize the reaction of 5-(2-carboxyphenyl)azo-8-quinolinol with uranium in aqueous medium at pH 5.2–6.1 to form a wine red coloured complex. The micellar sensitization results in two and a half-times enhanced molar absorptivity enabling the determination of uranium in rock samples at ppm level, stability of the complex enhanced from 4 hours to at least 72 hours. Extraction of the complex is avoided making the procedure simple, rapid and easy in operation. The molar absorptivity and Sandell's sensitivity of the complex are 1.50·10⁴l·mol^–1·cm^–1 and 15.9 ng·cm^–2, respectively, at _max=568 nm. Beer's law is obeyed over the range 0–3.3 g·ml^–1 of uranium. An amount as low as 0.19 g·ml^–1 of uranium could be determined satisfactorily within a relative standard deviation of ±1.3%. The limits of determination and practical quantitation are 0.29 and 1.80 ppm, respectively. The method was applied to the determination of uranium in soil, stream sediment and rock samples.     

Identification of metabolites in coriander seeds (Coriandrum Sativum L.) aided by ultrahigh resolution total correlation NMR spectroscopy

NMR is a fast method for obtaining a holistic snapshot of the metabolome and also offers quantitative information without separating the compounds present in a complex mixture. Identification of the metabolites present in a plant extract sample is a crucial step for all plant metabolomics studies. In the present work, we used various two dimensional (2D) NMR methods such as J-resolved NMR, total correlation spectroscopy (TOCSY), and heteronuclear single quantum coherence sensitivity enhanced NMR spectroscopy for the identification of 36 common metabolites present in Coriandrum sativum L. seed extract. The identified metabolites belong to the following classes: organic acids, amino acids, and carbohydrates. ¹H NMR spectra of such complex mixtures in general display tremendous signal overlap due to the presence of a large number of metabolites with closely resonating multiplet signals. This signal overlapping leads to ambiguity in an assignment, and hence, identification of metabolites becomes tedious or impossible in many cases. Therefore, the utility of pure-shift proton spectrum along the indirect (F₁) dimension of the F₁-PSYCHE-TOCSY spectrum is demonstrated for overcoming ambiguity in assignment of metabolites in crowded spectral regions from Coriandrum sativum L. seed extract sample. Because pure-shift NMR methods yield ultrahigh resolution spectrum (i.e., a singlet peak per chemical site) along one or more dimensions, such spectra provide better identification of metabolites compared with regular 2D TOCSY where signal overlap and peak distortions lead to ambiguity in the assignment. Nine metabolites were unambiguously assigned by pure-shift F₁-PSYCHE-TOCSY spectrum, which was unresolved in regular TOCSY spectrum.     

Spin state selective coherence transfer: a method for discrimination and complete analyses of the overlapped and unresolved 1H NMR spectra of enantiomers

In general, the proton NMR spectra of chiral molecules aligned in the chiral liquid crystalline media are broad and featureless. The analyses of such intricate NMR spectra and their routine use for spectral discrimination of R and S optical enantiomers are hindered. A method is developed in the present study which involves spin state selective two dimensional correlation of higher quantum coherence to its single quantum coherence of a chemically isolated group of coupled protons. This enables the spin state selective detection of proton single quantum transitions based on the spin states of the passive nuclei. The technique provides the relative signs and magnitudes of the couplings by overcoming the problems of enantiomer discrimination, spectral complexity and poor resolution, permitting the complete analyses of the otherwise broad and featureless spectra. A non-selective 180 degrees pulse in the middle of MQ dimension retains all the remote passive couplings. This accompanied by spin selective MQ-SQ conversion leads to spin state selective coherence transfer. The removal of field inhomogeneity contributes to dramatically enhanced resolution. The difference in the cumulative additive values of chemical shift anisotropies and the passive couplings, between the enantiomers, achieved by detecting Nth quantum coherence of N magnetically equivalent spins provides enhanced separation of enantiomer peaks. The developed methodology has been demonstrated on four different chiral molecules with varied number of interacting spins, each having a chiral centre.     

N-Chlorosuccinimide as a versatile reagent for the sulfenylation of ketones: a facile synthesis of α-ketothioethers

The sulfenylation of ketones having α-hydrogens has been achieved using N-chlorosuccinimide (NCS) under mild reaction conditions to produce α-ketothioethers in excellent yields with high selectivity. The use of NCS makes this method quite simple, convenient and practical.     

Enantiomeric discrimination by double quantum excited selective refocusing (DQ-SERF) experiment

The differences in chemical shift anisotropies, dipolar couplings, and quadrupolar couplings of two enantiomers in the chiral liquid crystalline media are employed to visualize enantiomers. In spite of the fact that proton has high magnetic moment and is abundantly present in all the chiral molecules, 1H NMR is not exploited to its full potential because of severe overlap of unresolved transitions arising from long- and short-distance couplings. Furthermore, the two spectra from R and S enantiomers result in doubling of the number of observable transitions. The present study demonstrates the application of the selectively excited homonuclear double quantum (DQ) coherence correlated to its single quantum coherence of an isolated methyl group in a chiral molecule. The DQ dimension retains only the passive couplings within the protons of the methyl group while the long-distance passive couplings are refocused, removing the overlap of central transitions, and each enantiomer displays a doublet instead of a triplet unlike in regular selective refocusing experiment. The doublet separation being different for each enantiomer results in their discrimination. The cross section taken along the single quantum dimension pertaining to each transition in the DQ dimension provides the one-dimensional spectra for each individual enantiomer with the complete removal of the overlapped transitions from the other enantiomer. The experiment is robust, the pulse sequence is easy to implement, and the methodology has been demonstrated on different chiral molecules.     

Semi numerical solution of non-singlet Dokshitzer–Gribov–Lipatov–Altarelli–Parisi evolution equation up to next-to-next-to-leading order at small x

The non-singlet structure functions have been obtained by solving Dokshitzer–Gribov–Lipatov–Altarelli–Parisi (DGLAP) evolution equations in next-next-to-leading order (NNLO) in the small-x limit. Here a Taylor series expansion has been used to solve the evolution equations and we obtain the semi numerical solution. Results are compared with the Fermi Lab experiment E665 data and New Muon Collaboration (NMC) data.     

Possibility of weakly charged nonlinear solitary dust clouds near the continuum threshold of dust population in a quasi-neutral dusty plasma

Considering the Boltzmann response of the plasma ions and electrons and inertial dynamics of the charged dust grains, the possibility of very weak compressive soliton near the continuum limit of the dust population has been inferred. It is concluded that the behaviour of such coherent structures could be well described by the numerical analysis of the derived nonlinear classical energy integral equation for bounded solutions. These seem to be higher order dispersive structures within acoustic limit of the nonlinear turbulence. It is observed that the dust density enhancement beyond the continuum threshold causes regular increment in width and amplitude of the soliton structures. It is found that the soliton amplitude sensitively depends on the massive impurity’s population. These coherent structures could be visualized as weakly charged solitary dust clouds of finite extension (∼ plasma Debye length) within Boltzmann environment of plasma particles in their local surroundings. The seeding mechanism of such clouds may be attributed to some plasma instabilities driven by either internal or external free energy sources. Numerical analysis of the problem concludes that the experimental observations of such clouds could be possible in low density plasma regime. It is deduced that for plasma density ∼ 10⁶ cm^-3 at temperatures of a few electron volts and for micron to l0nm sized dust grains, the observation of such structures could be possible within wide range variability of the dust population density.     

Real time band selective F1‐decoupled proton NMR for the demixing of overlay spectra of chiral molecules

The small chemical shift dispersion and complex multiplicity pattern in proton NMR limit quantifications, for instance the determination of enantiomeric excess (ee) for an enantiomeric mixture. Herein, we present a simple proton–proton correlation experiment with band selective homonuclear (BASH) decoupling in both F₁ and F₂ dimensions, for the removal of scalar and residual dipolar couplings to provide collapsed singlet for each chemical site. The method has been demonstrated to separate the severely overlapped spectra of enantiomers using both chiral isotropic and anisotropic phases as well as a small biomolecule, particularly for the diastereotopic protons and also for the determination of ee. Copyright © 2016 John Wiley & Sons, Ltd.     

Hybrid density functional∕molecular mechanics studies on activated adsorption of oxygen on zeolite supported gold monomer

Density functional theory calculations on oxygen adsorption over gas phase and faujasite supported Au monomer has been studied using hybrid quantum mechanics∕molecular mechanics method, surface integrated molecular orbital molecular mechanics implemented in GAMESS package. Three different oxidation states of Au (0, +1, +3) and three different adsorption modes viz., top, bridge, and dissociative adsorption of oxygen have been considered in our calculations. Redshift in the ν(O-O) value from that in gas phase O(2) indicates activation of O(2) upon adsorption over faujasite supported gold monomer. The activation of O(2) is an important step in the catalytic oxidation of CO. The presence of adsorbed O(2) increases the interaction of the Au monomer with the faujasite support. In faujasite supported cationic Au monomer, O(2) preferably remains bridge bonded to Au rather than being dissociated.     

Catalytic Activities of Au6, , and Clusters for CO oxidation: A density functional study

We have studied the CO oxidation over neutral, anionic, and cationic gold hexamer clusters using density functional theory which elucidates the effect of cluster charge state on the catalytic activity. Herein, we have considered the conventional bimolecular Langmuir–Hinshelwood mechanism with coadsorbed CO and O₂ at the neighboring sites in all the clusters. Among the three clusters, entails lower barriers during the various steps of the oxidation mechanism. The stability of all the species including the transition states with respect to the interacting species in indicates no thermal activation. Our study suggests better catalytic activity of as compared to the neutral and cationic counterparts. © 2014 Wiley Periodicals, Inc.