Sequencing of tri- and tetraantennary N-glycans containing sialic acid by negative mode ESI QTOF tandem MS

Application of the negative mode electrospray ionization-quadrupole time-of-flight mass spectrometry (ESI QTOF) tandem MS for determination of substitution patterns by sialic acid and/or fucose and extention by additional LacNAc disaccharide units in single branches of multianternary N-glycans from biological samples is described. Fragmentation patterns which can be obtained by low energy collision-induced dissociation (CID) using the QTOF instrument include cleavage ions, diagnostic for determination of antennarity and for specific structural features of single antennae. Systematic fragmentation studies in the negative ion mode were focussed toward formation of the D diagnostic ion relevant for assignment of 3- and 6-antennae in complex N-glycans carrying three and four antennae in combination with epitope-relevant B- and C-type ions. For validation of this approach ESI QTOF fragmentation of the permethylated analogues was carried out in the positive ion mode. Using this strategy, products of in vitro glycosylation reactions were investigated in order to clarify some general aspects of N-glycan acceptor specificity during biosynthesis. Alpha1-3fucosylation using GDP-fucose along with a soluble form of the recombinant human alpha1-3fucosyltransferase VI was carried out on tri- and tetraantennary precursors to test structural requirements for formation of Le(x) versus sLe(x) motifs.     

Auger-rekombination in Si

The Auger-recombination coefficient is determined to be C_n = 1,7·10^?31 cm⁶ sec^?1 and C_p = 1,2·10^?31 cm⁶ sec^?1 in Si. The Auger-recombination is not momentum conserving.     

Two-electron band to band transitions in silicon

Radiative two-electron band to band transitions are investigated experimentally in silicon. The question of phonon-participation is investigated. It turns out that at low temperature the phononless transitions dominate, whereas above room-temperature the phonon-assisted transitions become more important. A theoretical discussion leads to the conclusion that at least one participating phonon is an acoustical one.     

Indirekte Band-Band-Stoßrekombination in Germanium

Photoemission from Ge, caused by carrier injection via ap-n-junction was observed at a wavelength about 1 μm at room temperature. The dependence on energy, temperature and carrier density shows, that there appears a high energy tail of a radiative band to band recombination, which cannot be the tail of the usual radiative recombination in Ge. The intensity of the photoemission is proportional ton ² p ². The experimental results are explained as follows: Via indirect band to band Auger-recombination a hole is brought into the split-off band. In this band, the hole reaches thermal equilibrium with the lattice. While it remains in this band, it is able to recombine radiatively with electrons of the conduction band.     

A weight-function approach for determining watershed initial conditions for combustion waves

In many generic combustion models, one finds that a combustionwave will develop with a specific wave speed. However, thereare possible initial temperature profiles which do not evolveinto such waves, but rather die out to the ambient temperature.There can exist, in some models, a clear distinction betweenthose initial conditions that do evolve into combustion wavesand those that do not; this is sometimes referred to as thewatershed initial condition. When fuel consumption is consideredto be negligible, analytical methods can be used to obtain theexact watershed. In this paper, we consider the problem of determiningpseudo-watersheds and ascertaining the relationship betweenthese pseudo-watersheds and the exact watersheds. In the processa novel weight-function approach for infinite spatial domainsis developed.