排序方式: 共有6条查询结果,搜索用时 15 毫秒
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A. V. Bacheva O. V. Korobkina P. S. Nesterova V. A. Kryachkov A. G. Gabibov 《Moscow University Chemistry Bulletin》2016,71(2):97-103
Purification of the 11S proteasome regulator from mouse brain was optimized; the subunit composition of the isolated protein was determined by Western blot. The dependency of 20S proteasome peptidase activity on the molar concentration of the 11S regulator was examined. The Michaelis constants of hydrolysis of the specific fluorescent substrates Suc–Leu–Leu–Val–Tyr–AMC, Ac–Arg–Leu–Arg–AMC, and Z–Leu–Leu–Glu–AMC by the 20S proteasome from BALB/c mouse brain and the 20S–11S complex were determined. It was shown that the 11S particle has almost no influence on binding of specific fluorescent substrates to the 20S proteasome, but strongly accelerates hydrolysis of all three substrates, while not affecting the rate of peptide substrate hydrolysis by the 26S proteasome. 相似文献
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Filippova I. Yu. Bacheva A. V. Baibak O. V. Plieva F. M. Lysogorskaya E. N. Oksenoit E. S. Lozinsky V. I. 《Russian Chemical Bulletin》2001,50(10):1896-1902
Covalent immobilization of subtilisin and thermolysin on cryogel of poly(vinyl alcohol) was carried out. The biocatalysts obtained are characterized by high stability in water and in DMF—MeCN mixtures of various compositions. The synthetic efficiency of immobilized subtilisin in the multiple iterative synthesis of the peptide Z—Ala—Ala—Leu—Phe—pNA was examined in organic mixtures of different solvent compositions. Immobilized subtilisin exhibits high synthetic activity in organic media. A series of N-acylated p-nitroanilides of tetrapeptides of the general formula Z—Ala—Ala—Xaa—Yaa—pNA (Z is benzyloxycarbonyl, Xaa = Leu, Lys, or Glu; Yaa = Phe or Asp; pNA = 4-NO2—C6H4NH-) were synthesized in 70—98% yields using immobilized subtilisin as a biocatalyst without activation and protection of the ionogenic groups of polyfunctional amino acids. Immobilized thermolysin in a DMF—MeCN mixture catalyzed the formation of the peptide Z—Ala—Ala—Leu—pNA, which was obtained in 90% yield (during 1 h). It was demonstrated that the biocatalyst can be used repeatedly and that it retained activity after storage in an aqueous buffer during 6 months. 相似文献
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Vesna Bacheva Dr. Federico Paratore Dr. Shimon Rubin Dr. Govind V. Kaigala Prof. Moran Bercovici 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(31):12994-12999
We present a new concept for on-chip separation that leverages bidirectional flow, to tune the dispersion regime of molecules and particles. The system can be configured so that low diffusivity species experience a ballistic transport regime and are advected through the chamber, whereas high diffusivity species experience a diffusion dominated regime with zero average velocity and are retained in the chamber. We detail the means of achieving bidirectional electroosmotic flow using an array of alternating current (AC) field-effect electrodes, experimentally demonstrate the separation of particles and antibodies from dyes, and present a theoretical analysis of the system, providing engineering guidelines for its design and operation. 相似文献
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Vesna Bacheva Federico Paratore Shimon Rubin Govind V. Kaigala Moran Bercovici 《Angewandte Chemie (International ed. in English)》2020,59(31):12894-12899
We present a new concept for on‐chip separation that leverages bidirectional flow, to tune the dispersion regime of molecules and particles. The system can be configured so that low diffusivity species experience a ballistic transport regime and are advected through the chamber, whereas high diffusivity species experience a diffusion dominated regime with zero average velocity and are retained in the chamber. We detail the means of achieving bidirectional electroosmotic flow using an array of alternating current (AC) field‐effect electrodes, experimentally demonstrate the separation of particles and antibodies from dyes, and present a theoretical analysis of the system, providing engineering guidelines for its design and operation. 相似文献
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V. A. Kryachkov V. N. Tashlitsky A. V. Bacheva 《Moscow University Chemistry Bulletin》2018,73(4):166-172
Fluorogenic polyglutamine-containing peptides with five and ten glutamine residues in a row, having a FRET pair of EDANS (fluorophore) and Dabcyl (quencher), are characterized using spectral and mass spectrometric methods. The possibility of their hydrolysis by the 20S proteasome is examined. The kinetic parameters (catalytic efficiency) for these substances are determined. The presence of glycine in the substrate significantly decreases the solubility of the substrate and diminishes the efficiency of hydrolysis with the proteasome. 相似文献
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A. S. Malinin A. A. Rakhnyanskaya A. V. Bacheva A. A. Yaroslavov 《Polymer Science Series A》2011,53(1):52-56
The immobilization of α-chymotrypsin on the surface of boron silicate glass microspheres is conducted via the technique of
multilayer adsorption of polyelectrolytes. It is shown that the enzyme is adsorbed on both positively and negatively charged
surfaces and its activity is partially preserved relative to that in solution. The activity of the enzyme depends on the number
of polyectrolyte layers preliminarily adsorbed on glass microspheres and on the charge of the surface. The activity of α-chymotrypsin
adsorbed on the negatively charged surface is four times higher than the activity of this enzyme adsorbed on a positively
charged surface. 相似文献
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