Synthesis of Some N-Alkoxycarbonyl-N″-benzoyl-benzamidrazones（p-toluamidrazones） and 1,3,5-Trisubstituted 1,2,4-Triazole Derivatives from N-Benzoylimidates and their Antimicrobial and Anticancer Screening Studies

Some new N-alkoxycarbonyl-N″-benzoyl-benzamidrazones （p-toluamidrazones） 3a-3d, and 1,3,5-trisubstituted 1,2,4-triazole 4a-4h derivatives by starting from N-benzoylbenzimidates or N-benzoyl-p-toluimidates. The structures of compounds 3 and 4 were established on the basis of elemental analyses, IR, ^1H NMR, ^13C NMR and UV data. Antimicrobial experiments of the compounds performed by using agar-well diffusion and broth microdilution methods revealed that only compounds 3a-3d, 4a and 4b showed inhibitory effect only on Candida albicans ATCC 60193. However, compound 4b had also specific antibacterial activity against Staphylococcus aureus ATCC 25923. The other compounds showed neither antifungal nor antibacterial activities. Compounds 3a, 4a and 4b have been screened on three human tumor cell lines, breast cancer （MCF7）, non small cell lung cancer （NCI-H460）, and CNS cancer （SF-268） at the National Cancer Institute （NCI）, USA, which were found to exhibit low antiproliferative activity.     

A New pH Indicator Based on 2,5‐Diaryl‐1‐salicylideneamino‐1,3,4‐triazole Derivative

2‐Phenyl‐5‐p‐tolyl‐1‐salicylideneamino‐1,3,4‐triazole (PTST) was studied among 2,5‐diaryl‐1‐salicylidene amino‐1,3,4‐triazole derivatives as a novel potential pH indicator. In terms of absorption intensity coming from the acid‐base reactions, PTST showed strong yellow‐green colour with high extinction coefficient in the pH range 7.0–9.5. In addition, the corresponding colour development at the transition point can be attributed to the resonance structures of PTST, which were caused by the hydrogen ion dissociation from the acidic form of the compound in the presence of alkali. The full geometric optimization and achievement of the electronic structure of the molecule were performed by an AM1 semiempirical method. The triazole compound was compared with phenolphthalein (PT), which is widely used as an acid‐base indicator in titrimetry, for accuracy test.