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High-performance liquid chromatographic determination of isepamicin in plasma, urine and dialysate 总被引:2,自引:0,他引:2
A high-performance liquid chromatographic method for the measurement of isepamicin, a new aminoglycoside, in plasma, urine and dialysate is reported. The assay utilizes a simple extraction of isepamicin in plasma using commercially available Cyano solid-phase cartridges and dilution of urine and dialysate samples. The separation is performed on a Hypersil C18 column (15 cm X 4.6 mm I.D., 5 microns particle size) and utilizes a mobile phase consisting of 10% methanol and 90% buffer solution containing 0.01 M sodium hexanesulfonate, 0.1 M sodium sulfate and 17 mM acetic acid. The flow-rate is 1.1 ml/min. Dibekacin is used as the internal standard. Isepamicin is derivatized post-column with o-phthalaldehyde for spectrofluorometric detection. The method can also be used for the measurement of other aminoglycosides, i.e. tobramycin, kanamycin, netilmicin and gentamicin. The assay is fast, accurate and has a quantitation limit of 100 ng/ml isepamicin in plasma and 50 ng/ml in urine and dialysate. 相似文献
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The intermolecular interaction between poly(methyl methacrylate) (PMMA) and polystyrene (PS) intetrahydrofuran (THF) and N,N'-dimethyl formamide (DMF) solvents was studied at 28℃ using a dilute solution viscometrymethod. Solvent is believed to play a key role in characterizing the viscosity behavior of the polymer solution. The intrinsicviscosity and viscosity interaction parameter were experimentally measured for the binary (solvent/polymer) and for theternary systems in two solvents. The compatibility of the polymer mixture was discussed in terms of the sign of △b_m. Theresults show that the compatibility of PMMA/PS blend in DMF is larger than that in THF. 相似文献
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An attempt to link the heat of reaction obtained from analysis of UV spectra, bond energies deduced from crystallographic
data and calorimetric measurements of [bis(d-, l-, and dl-alaninato)diaqua] nickel(II) dihydrate complexes to theoretical values obtained from calculation is described. The heat of
reaction, which includes energy of ligand exchange in addition to crystal field stabilization energy of the three complexes,
was calculated from changes in spectral shifts, bond lengths, and free energy between nickel(II) ion and the d-, l-, and dl-alanine complexed nickel(II) ion in aqueous solution. These investigations were done by UV–visible spectroscopy, X-ray crystallography,
and isothermal calorimetry. The results reveal that the experimental heat of reaction based on the three mentioned parameters
is in good agreement with the theoretical value. The results are found to be of considerable interest in its linking of spectrochemical
quantities with thermodynamic functions. 相似文献
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<正>The origin of homochirality in living organisms is controversial,stands out of being particularly important and a question which is still not satisfactorily answered.A mental picture of sequence of events that is thought to have preceded the existence of chirality in molecules is described.A chemical model to mimic the original abiotic conditions in an attempt to explain the preference of homochirality in living systems was tried.The effect which might have influenced this preference is presented.The surprising and unexpected results are indeed interesting,significant,repeatable and indicate that complexing alanine with nickel(Ⅱ) ion alters the racemization rates of D and L isomers of the amino acid.However,why this difference happens is unclear and is difficult to explain. 相似文献
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