Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products

The present study describes the possibilities offered by an innovative bioinert size exclusion chromatography column for size variant characterization of complex monoclonal antibody products. This size exclusion chromatography column includes a novel column hardware surface. The column was prepared from metallic hardware components that were treated to have prototype hydrophilically modified hybrid organic–inorganic silica surfaces called hybrid surface technology. This provides a significant reduction in nondesired hydrophobic and electrostatic interactions that can occur between column and analyte when performing size exclusion chromatography analysis with volatile mobile phase. Compared to a reference stainless-steel column packed with the same batch of packing material, peak tailing, band broadening, and above all recovery of high molecular weight species were distinctly improved for all types of monoclonal antibody products. Based on our observations, we found that 50 mM ammonium acetate in water was a suitable mobile phase offering good compromise in terms of liquid chromatography performance and mass spectrometry sensitivity. In addition, method repeatability (intra- and interday relative standard deviations) on elution times and high molecular weight species peak areas were found to be excellent. By using this innovative size exclusion chromatography material, the low and high molecular weight species contained in various stressed and nonstressed monoclonal antibody products were successfully characterized with mass spectrometry detection.     

Therapeutic Fc‐fusion proteins: Current analytical strategies

Fc‐Fusion proteins represent a successful class of biopharmaceutical products, with already 13 drugs approved in the European Union and United States as well as three biosimilar versions of etanercept. Fc‐Fusion products combine tailored pharmacological properties of biological ligands, together with multiple functions of the fragment crystallizable domain of immunoglobulins. There is a great diversity in terms of possible biological ligands, including the extracellular domains of natural receptors, functionally active peptides, recombinant enzymes, and genetically engineered binding constructs acting as cytokine traps. Due to their highly diverse structures, the analytical characterization of Fc‐Fusion proteins is far more complex than that of monoclonal antibodies and requires the use and development of additional product‐specific methods over conventional generic/platform methods. This can be explained, for example, by the presence of numerous sialic acids, leading to high diversity in terms of isoelectric points and complex glycosylation profiles including multiple N‐ and O‐linked glycosylation sites. In this review, we highlight the wide range of analytical strategies used to fully characterize Fc‐fusion proteins. We also present case studies on the structural assessment of all commercially available Fc‐fusion proteins, based on the features and critical quality attributes of their ligand‐binding domains.     

Measurement of the thermal lens of grazing-incidence diode-pumped Nd:YVO4 laser amplifier

The thermally induced lensing effects of a diode-pumped Nd:YVO₄ laser amplifier in a grazing incidence bounce geometry are carefully measured experimentally. Measurements of the thermal effects are interpreted by considering the diode-pumped amplifier region as a thick lens. A more detailed modeling was made of a quadratic refractive index lens duct for the normal to the bounce plane distribution. Due to the asymmetric pumping the thermal lens shows significant astigmatism. The ratio between dioptric powers in the plane normal to bounce and in the bounce plane is measured to be approximately 20. A nonlinear component of the power dependence of the lens is observed and related to the nonlinear heating induced by energy transfer upconversion. Measurements made under non-lasing conditions are used to infer thermal lensing behavior under lasing conditions and this allows optimization of optical resonator design.     

Propagation of partially coherent beams: turbulence-induced degradation

We show that, when a partially coherent beam propagates through an inhomogeneous medium such as atmospheric turbulence, the phase randomization that is induced is less effective in degrading the spatial coherence properties. By evaluating the final beam widths we report what is to our knowledge the first experimental demonstration that, on propagation through thermlly induced turbulence, a partially coherent beam is less affected than a spatially coherent beam.