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Vinod K. Ahirrao Rajiv A. Jadhav Suyog N. Vaidya Sachin S. Bhagwat Ravindra D. Yeole Mahesh V. Patel 《Biomedical chromatography : BMC》2022,36(6):e5354
Antibiotic susceptibility test (AST) discs are used as an in-vitro diagnostic tool to select the appropriate antibiotic to treat an infection. Generally, the concentration of the drug loaded on to the AST discs is measured by studying its activity against quality control organisms. This methodology has several limitations—it is time consuming, requires trained manpower, has a wider acceptance criteria of zone of inhibitions—causing ambiguity in judging smaller variations in drug concentration. To overcome these issues, we have developed and validated high-performance liquid chromatographic (HPLC) methods for the determination of strength of AST discs for in-house researched antibiotics, namely Levonadifloxacin/WCK 771, Nafithromycin/WCK 4873, Cefepime-Tazobactam/WCK 4282, and Cefepime-Zidebactam/WCK 5222. The drugs were extracted from the AST discs using an appropriate solvent. The developed methods are simple, accurate, precise, reproducible, rugged, and robust. They are efficient in terms of time, and can be easily conducted in a quality control laboratory during release as well as stability evaluation of AST disc. Application of HPLC methods for the determination of strength of AST discs ensures flawless quality and, consequently, a better selection of drugs to treat bacterial infections in clinics. 相似文献
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Rane Vipul P. Patil Rushikesh H. Patil Kiran R. Pathan Amin R. Naik Sukantakumar Ahirrao Vinod K. Jadhav Rajiv A. Yeole Ravindra D. 《Chromatographia》2022,85(2):155-165
Chromatographia - WCK 771 is a novel antibacterial drug recently launched in India for the treatment of acute bacterial skin and skin structure infections (ABSSSI). This report describes... 相似文献
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N. V. Bhat S. M. Ahirrao 《Journal of polymer science. Part A, Polymer chemistry》1983,21(5):1273-1280
The Silk fibers of the mulberry (Bombyx Mori) were dissolved in a 70% lithium thiocyanate solution. Dissolved silk was regenerated by casting the films from the solution after dialyzing. The films were investigated by infrared (IR) spectroscopy and x-ray diffraction analysis. It was found that the freshly prepared film was amorphous. The transformation to the β-form could be brought about by heating, solvent induced crystallization, ultraviolet (UV) radiation, and prolonged storage. The mechanism of this transformation is discussed. 相似文献
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Ravindra D. Yeole Vipul P. Rane Vinod K. Ahirrao Rajesh P. Chavan Anasuya M. Patel Prasad K. Deshpande Mahesh V. Patel Kiran R. Patil 《Biomedical chromatography : BMC》2019,33(7)
WCK 771 is an l ‐arginine salt of levonadifloxacin (LND) being developed in intravenous dosage form and has recently completed a phase III trial in India. The pharmacokinetics of WCK 771, a novel anti‐MRSA fluoroquinolone, were examined in mice, rats, rabbits, dogs, monkeys and humans after systemic administration during pre‐clinical and clinical investigations. Urine and serum were evaluated for identification of metabolites. It was observed that LND mainly follows phase II biotransformation pathways. All of the species showed a different array of metabolites. In mice, rabbit and dog, the drug was mainly excreted in the form of O‐glucuronide (M7) and acyl glucuronide (M8) conjugates, whereas in rat and human major metabolite was sulfate conjugate (M6). Monkeys exhibited equal distribution of sulfate (M6) and glucuronide conjugates (M7, M8). In addition to these three major phase II metabolites; five phase I oxidative metabolites (M1, M2, M3, M4 and M5) were identified using liquid chromatography tandem mass spectrometry. Out of these eight metabolites M2, M3, M5, M7 and M8 are reported for the first time. 相似文献
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Vinod Ahirrao Vipul Rane Kiran Patil Rajiv Jadhav Anasuya Patel Vijay Patil Ravindra Yeole 《Biomedical chromatography : BMC》2019,33(9)
A chiral liquid chromatographic method was developed and validated for the quantification of R‐enantiomer impurity (RE) in WCK 3023 (S‐enantiomer), a new drug substance. The separation was achieved on Chiralpak IA (amylose‐based immobilized chiral stationary phase), using a mobile phase consisting of n‐hexane–ethanol–trifluoroacetic acid (70:30:0.2, v/v/v) at a flow rate of 1.0 mL/min. The method was extensively validated for the quantification of RE in WCK 3023 and proved to be robust. For RE the detector response was linear over the concentration range of 0.11–5 μg/mL. The limit of quantitation and limit of detection for RE were 0.11 and 0.04 μg/mL respectively. Average recovery of the RE was in the range of 98.11–99.55%. The developed method was specific, sensitive, precise and accurate for quantitative determination of RE in WCK 3023. The impact of thermodynamic parameters on the chiral separation was evaluated. The method was employed for controlling the enantiomeric impurity in the lots of WCK 3023 used for pre‐clinical studies. The method was successfully applied to evaluate the possible conversion of WCK 3023 to RE in rat serum samples during pre‐clinical pharmacokinetic studies. 相似文献
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