致瘤农杆菌能够转化大麦和小麦

直到现在,单子叶植物特别是禾谷类作物的基因工程还没有合适的载体系统。本文首次报道了致瘤农杆菌T37,A208和B63个菌种能够感染大麦和小麦的部分品种,形成突起和肿瘤。并且发现,酚类化合物乙酰丁香酮对转化有促进作用。另外,在合适的植物组织接种农杆菌也是转化成功的关键。文中讨论了农杆菌的宿主范围以及Ti质粒作为禾谷类作物基因工程载体的可能性。     

EXPRESSION OF A ZEIN GENE (Z4) INTRODUCED INTO DICOTYLEDONOUS Solanum nigrum

A maize genomic clone containing a zein gene (Z4) is inserted into the T-DNA of the Ti plasmid pTiT37. Agrobacterium tumefaciens strain harboring this modified Ti plasmid is used to infect stem sections of young plants or explants of dicotyledonous Solanum nigrum. Axenic transformed calli active in nopaline synthesis are obtained and transgenic plants are differentiated from them DNA Southern hybridization and RNA dot-hybridization analyses show that the zein gene is really transferred and integrated into the nuclear genome of transformed Solanum nigrum and that the zein gene can be transcribed into mRNA in the transformed calli and shoots. But the presence of the zein protein cannot be detected in either the transformed calli or the transgenic shoots. The results of thte experiments demonstrate that the promoter of a gene from monocotyledonous plants can function normally in transgenic dicots. The possibility of developmentally-regulated expression of the zein gene in transformed dicots is discussed in     

玉米醇溶蛋白基因Z_4在双子叶植物龙葵(Solanum nigrum)中的表达

本文将含有玉米醇溶蛋白基因Z_4的玉米基因组克隆插入Ti质粒pTiT_(37)的T-DNA区。用带有此质粒的根癌农杆菌感染龙葵,得到了能合成胭脂碱的转化愈伤组织和转化植株。DNA分子杂交和RNA点杂交表明,Z_4基因确实转化并整合进了龙葵的核基因组,并且被转录成mRNA。但在转化体中,没能检测出玉米醇溶蛋白。实验证明,单子叶植物基因的启动子能够在双子叶植物中发挥功能。文中讨论了Z_4基因在双子叶转化植物的种子中受发育调节的可能性。     

Agrobacterium tumefaciens CAN TRANSFORM Triticum aestivum AND Hordeum vulgare OF GRAMINEAE

So far there is no suitable vector system for the genetic engineering of monocotyledonous plants, especially cereal crops. It is first reported in this paper that Agrobacterium tumefaciens strains T37, A208 and B6 can transform some varieties of Triticum aestivum and Hordeum vulgare to form swellings and tumors. Itis shown in the experiments that phenolic compound acetosyringone may promote the transformation process. In addition, inoculating agrobacteria on appropriate plant tissues is also proved to be the key step in achieving successful transformation. The host range of A. tumefaciens and the possibility of utilizing Ti plasmid as the vector for the gene transfer of cereal crops are discussed.