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本文应用化学降解法测定了adr亚型乙型肝炎病毒pADR-1 DNA中包含表面抗原基因(s基因)的XhaI BamHI片段顺序,共1279碱基对。比较了adr亚型与已知的adw,adyw,ayw的s基因的核苷酸顺序及其编码的HBsAg氨基酸顺序的差异,发现了一些新的变异位点,差异集中分布在HBsAg的亲水区域。但在一些可能具有生物功能的位点,各亚型间并无变异,在遗传和进化中是相对保守的。比较s基因区和非s基因区的核苷酸变异情况表明,非s基因区的变异频率高一倍,s基因区是相对保守的。 相似文献
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本文报道利用HBVadr DNA的Hind Ⅲ切点,将HBV DNA切开,插进pBR322的Hind Ⅲ位点,获得12株带HBVadr基因组的克隆株。限制性酶切分析,发现pADR-H1虽然也只有一个Hind Ⅲ切点,但它在基因组中的位置与pADR-1不同。pADR-1的HBV DNA中,其Hind Ⅲ-Bam HI片段的大小为315 bp,而pADR-H1中的相应片段只有82bp。序列分析表明,前者82 bp处的一段AAGTTT结构,在pADR-H1中变为AAGCTT而成了Hind Ⅲ识别位点。此外,限制性内切酶AvaⅠ,HincⅡ,HpaⅠ和SphⅠ对二克隆株的作用情况也有差别。本文探讨了这些差别的意义。 相似文献
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We have cloned the HBV genome subtype adr through its HindⅢ site into plasmid pBR.322. Twelve recombinant plasmids each with an insert of the HBV genome were obtained. The restriction map of one of the recombinants, plasmid pADR-H1, was analyzed. The location of the sites for BamHⅠ, BglⅠ, BglⅡ, SstⅡ, XbaⅠ and XhoⅠ in pADR-HⅠ were found to be the same as that in pADR-1, but the HindⅢ site of pADR-H1 is distinctly different from that of pADR-1. The BamHⅠ-HindⅢ fragment is 316 bp long in the gcnome of pADR-1. However, it is only 82 bp in pADR-Hl. No deletion of sequence between BamHⅠ and HindⅢ sites has been found in the HBV genome of pADR-H1, The sequencing data around the HindⅢ site of pADR-H1 in both pADR-H1 and pADR-1 show that there is a stretch of AAGTTT in pADR-1 compared to an AAGCTT in pADR-H1. In addition, other differences were found. There are three sites for AvaⅠ, four for HincⅡ, one for HpaⅠ and none for SphⅠ in the genome of pADR-H1 compared to four sites for AvaⅠ, three f 相似文献
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THE COMPLETE NUCLEOTIDE SEQUENCE OF THE CLONED DNA OF HEPATITIS B VIRUS SUBTYPE adr IN pADR-1 总被引:4,自引:0,他引:4
The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 wasdetermined by Maxam and Gilbert's method. It is 3215 base pairs in size, which is 27 bp longer thanthe sequence of the adr pHBr330, as reported by Ono et al. The nucleotide difference between pADR-1and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7%respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the othercoding regions in pADR-1 and in the other subtypes are compared and discussed. 相似文献
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The nucleotide sequence of the XhoI-BamHI fragment (1279bp), which contains the sur-face antigen gene (S gene) of HBVadr, was determined by Maxam and Gilbert's method.By comparing the differences both of the nuclectide sequence in the S gene and its codedamino acid sequence between adr and those reported for adw, ayw and adyw, some new var-iation sites were discovered. The differences were mainly distributed in the two hydrophilieregions. However, at those sites which might show biological function, there were no varia-tions among different subtypes, they are relatively conservative in heredity and evolution.Comparing the variation of the nucleotide sequence in the S gene region with that in thenon-S gene region, it is shown that the frequency of variation in the non-S gene regiondoubled that in the S gene region. Tim S gene region is more conservative. 相似文献
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