大麦条纹花叶病毒在大麦植株内运转的研究

本文按常规方法制备感染有大麦条纹花叶病毒(BSMV)的大麦叶片超薄切片中,很容易观察到薄壁细胞内的BSMV颗粒。但病叶的筛管、导管和胞间连丝中从未观察到该病毒颗粒。未标记免疫酶技术的研究表明:病叶筛管里毫无例外地存在着大量的BSMV蛋白;在幼嫩的木质部和胞间连丝中,也常常能观察到BSMV蛋白;有时成熟的导管中亦充满着BSMV蛋白。这说明在BSMV所侵染的大麦植株中BSMV蛋白与植株内正常的代谢物质一样,经筛管、导管和胞间连丝转移。基于这些结果作者讨论了大麦条纹花叶病毒以核酸的形式在植林内运转的可能性。     

STUDY ON TRANSLOCATION OF BARLEY STRIPE MOSAIC VIRUS WITHIN DISEASED BARLEY PLANT

It was easy to observe the particles of barley stripe mosaic virus (BSMV) in parenchyma cells in the ultrathin sections prepared from infected barley leaves according to routine methods. But no particles of BSMV were seen in phloem xylem and plasmodesmata. The research work in which the unlabelled immunoperoxidase technique was employed indicated that there was always a large amount of BSMV proteins in sieve tubes of diseased leaves. BSMV proteins were often seen in young xylem and plasmodesmata, occasionally mature xylem was also filled with them. All these facts sugest that BSMV proteins are translocated through phloem, xylem and plasmodesmata in barley plants infected with BSMV in the same way in which normal metabolic materials are translocated in healthy barley plants.The possibility that BSMV is translocated within the diseasod barley plant in the form of nucleic acid is discussed.