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The oxidase-like activity of cobalt oxyhydroxide (CoOOH) nanosheets is inhibited by arsenic (V) in the system, thereby attenuating the oxidation of p-phenylenediamine (PPD) by CoOOH nanosheets, and changing the absorbance of the solution. Based on this principle, and the fluorescence inner filter effect (IFE) of p-phenylenediamine oxide (oxPPD) on the dye acridine orange (AO), a colorimetric and fluorescence dualmode method for the quantitative detection of As(V) was established. The variation of absorbance and fluorescence intensity of As(V) with different concentrations was investigated, and the effects of the concentrations of CoOOH nanosheet and PPD, pH of buffer solution, and temperature of reaction were optimized. The results showed that under the optimized conditions, the concentration of As(Ⅴ) had a good linear relationship with the corresponding signal intensity, and the detection limits of colorimetric and fluorescence method were 5 and 10 nmol/L, respectively. The method has been used for the determination of arsenic(V) in lake water located the city center. © 2023, Youke Publishing Co.,Ltd. All rights reserved.  相似文献   
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