北京鸭肝脏线粒体DNA的限制酶图谱

本文用五种限制性内切酶(EcoRⅠ,BamHⅠ,PstⅠ,BglⅠ和BglⅡ)建立了北京鸭肝脏线粒体DNA的内切酶图谱。这五种酶除BglⅡ不能切割鸭肝线粒体DNA外,EcoRⅠ,BamHⅠ,PstⅠ和BglⅠ在鸭肝线粒体DNA上分别有1,2,4和5个切割位点。应用电子显微镜及电泳法对鸭肝线粒体DNA的各种酶解片段进行测定后,推导出了此线粒体DNA的限制酶图谱。此外,还确定了D-环的位置及线粒体DNA复制的方向。     

RESTRICTION ENDONUCLEASE CLEAVAGE MAP OF MITOCHONDRIAL DNA FROM PEKING DUCK LIVER

Mitochondrial DNA of the liver from Peking duck was cleaved by restricition endonu-cleases EcoRI, BamHI, PstⅠ and BglⅠ into 1, 2, 4 and 5 fragments, respectively, while the BglⅡ was without any cleavage. The restriction map of this mtDNA was constructed by measuring the length of restriction fragments using both electrophoresis analysis and electron microscopy. The position of D-loop and the direction of replication of the mtDNA were also determined.