STUDY ON INDUCTION OF DIFFERENTIATION OF A HUMAN MEGAKARYOBLASTIC LEUKEMIA CELL LINE (HIMeg) BY 1,25-DIHYDROXYVITAMIN D_3

It is reported that 1,25-dihydroxyvitamin D_3(1,25(OH)_2D_3), a physiological factor, has aninductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line(HIMeg) in vitro. At the concentrations ranging from 10~(-9) to 10~(-6) mol/L, 1,25(OH)_2D_3 showedinhibition of proliferation on HIMeg cells which was demonstrated by count of survivalcells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochem-istry (including immunoenzymatic technique) and flow cytometry, we found that HIMeg cellscould be further induced into more mature cells in megakaryocytic lineage confirmed by aseries of evidence, including the changes of cell morphology/structure and cytochemistry,increased expression of differentiation antigens on the cell surface, and polyploidization.So, it is possible for 1,25(OH)_2D_3 to promote the differentiation of the cells in megakaryo-cytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other di-seases with mal     

1,25(OH)_2D_3对人原始巨核白血病细胞系(HIMeg)的诱导分化研究

本文报道了生理性诱导剂1,25(OH)_2D_3对一株新建的人原始巨核白血病细胞系(HIMeg)的体外诱导分化作用。在浓度为10~(-9)—10~(-6)mol/L的1,25(OH)_2D_3作用下,活细胞计数、克隆形成率测定显示1,25(OH)_2D_3对HIMeg细胞有增殖抑制作用;同时,应用光/电子显微镜、细胞化学染色、免疫酶标、流式细胞分析等技术,从细胞形态结构、细胞化学、细胞表面分化抗原以及DNA倍体性诸方面证实了1,25(OH)_2D_3能诱导HIMeg细胞进一步分化成熟。从而提示:1,25(OH)_2D_3可能是体内巨核系的一个重要促分化因子,并可试用于治疗急性巨核细胞白血病等疾病。