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The E.coli leucyl-tRNA synthetase (E. C. 6. 1. 1. 4) was specifically labelled with 3'-oxidized tRNA~(Leu) (tRNA_(ox)~(Leu)).The procedure involves a Schiff's base formation and its subsequent reduction by sodium cyanoborohydride. Stoichiometric inactivation of aminoacylation was achieved with the incorporation of 1 mol of tRNA_(ox)~_(Leu) per mol LeuRS.On the other hand, the amino acid activation activity of LeuRS-tRNA_(ox) complex was partially inhibited. After extensive digestion of the complex by pancreatic ribonuclease, the amino acid activation activity was fully recovered, while the aminoacylation activity was not restored at all.The results suggest that the active sites responsible for the activities of LeuRS correlate with each other as well as differ from each other. 相似文献
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目的 探讨去甲斑蝥素对人胰腺癌PANC-1细胞凋亡的作用及其机制。方法 将PANC-1 细胞株随机分为处理组
和对照组,处理组加入不同浓度的去甲斑蝥素培养24h后,CCK-8 法检测细胞增殖情况;流式细胞术分析细胞的凋亡情况;免疫印迹法检测细胞内质网应激和凋亡相关蛋白的表达;荧光定量PCR 技术检测细胞内质网应激和凋亡相关蛋白mRNA表达。结果 不同浓度去甲斑蝥素处理PANC-1细胞24h后,与对照组相比,能降低细胞的存活率并诱导其凋亡。与对照组相比,处理组中内质网应激和凋亡相关蛋白的表达水平均上调(均P<0.05),同时其mRNA 的表达水平亦均上调(均P<0.05)。结论 去甲斑蝥素能明显抑制人胰腺癌PANC-1 细胞的生长并诱导其凋亡,并且具有浓度依赖性,这一作用可能是通过内质网应激介导的凋亡途径实现的。 相似文献
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本文用3′-末端氧化的tRNA~(Leu)(tRNA_(ox)~(Leu))专一地标记了大肠杆菌亮氨酰-tRNA合成酶(LeuRS)。合成酶标记上等摩尔tRNA_(ox)~(Leu)后,氨酰化活力完全丧失,同时氨基酸活化活力也失去80%。这样失活反应是通过tRNA_(ox)~(Leu)的3′-末端羰基与亮氨酰-tRNA合成酶活性中心或其附近的赖氨酸残基的ε-氨基形成Schiff碱实现的。Schiff碱经硼氢氰化钠还原后稳定。酶与tRNA_(ox)~(Leu)的共价复合物经牛胰核糖核酸酶充分水解后,其活化氨基酸的活力及动力学常数基本恢复到原来水平,而氨基酰化活力未恢复。表明了该合成酶的两种活性中心相互关联又有区别。 相似文献
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沉淀方法对铜基甲醇合成催化剂前驱体及其性能的影响 总被引:2,自引:0,他引:2
分别采用共沉淀法 (CP)、两步沉淀法 (TP) 和分步沉淀法 (FP) 制备了 Cu/ZnO/Al2O3 甲醇合成催化剂. 结果发现, FP 催化剂上甲醇收率比 CP 催化剂上高 46.3%, 比 TP 催化剂上高 9.3%. 采用 X 射线衍射、微分热重分析、红外光谱、N2 吸附-脱附、程序升温还原及 N2O 滴定等方法表征了催化剂. 结果表明, FP 催化剂前驱体中 (Cu,Zn)2CO3(OH)2 和 (Zn,Cu)5(CO3)2(OH)6 两种高活性物相较多, 而 (Cu,Zn)6Al2(OH)16CO3•4H2O 物相较少, 焙烧后形成了较多的 CuO-ZnO 固溶体, 同时形成了较多的碳酸盐, 因而催化活性更高. 相似文献
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针对核物理实验中高精度时间同步触发图像采集系统的要求,设计了一套基于FPGA和蓝牙的远程图像采集同步系统。该系统以FPGA作为主控制器,负责USB 接口通信、命令解析、蓝牙通信和同步控制等,触发信号延时精度可达10 ns;使用RS485 或TTL 标准以适应长距离触发和短距离触发,通过GigE 千兆网口实现远程图像数据采集。采用LabVIEW 开发了上位机图形化软件界面,基于蓝牙BLE4.0 协议,实现了基于手机APP的无线终端控制,完成了对相机延时时间、同步触发脉冲宽度、图像采集等命令的同步控制。A image acquisition and synchronization control system based on FPGA (Field Programmable Gate Array) and bluetooth is studied in this paper. FPGA as the main controller is responsible for USB (Universal Serial BUS) interface, command analyze, delay control, and trigger signal generation. Trigger signal can be RS485 or TTL. RS485 signals can be used for long-distance transmission without distortion while TTL is suitable for near field. The system's delay accuracy is reached to 10ns. It can be obtain images from GigE Ethernet port. Meanwhile,a PC graphical control software is developed by LabView. And based on the Bluetooth BLE4.0 protocol, a Android mobile phone APP is developed for wireless control. 相似文献
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The covalent modification of E. coli arginyl-tRNA synthetase by the 2',3'-dialdehydederivative of tRNA~(Arg) (tRNA_(ox)~(Arg)) resulted in the complete inactivation of the ATP-PPi ex-change and aminoacylation activities of the enzyme. Sodium dodecyl sulfate polyacrylamide gelelectrophoresis of the ArgRS-tRNA_(ox)~(Arg) covalent complexes indicated that two bands simulta-neously appeared on the gel parallel with inactivation corresponding to different higher mo-lecular weights. This result was different from that of the other aminoacyl-tRNA synthetaselabeling systems as previously reported. Upon the ribonuclease treatment of the modifiedArgRS, less than 15% of both the initial ATP-PPi exchange and aminocylation activities wererecovered. During the whole process of labeling and RNase treatment, the two activities ofthe enzyme were closely associated. 相似文献
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The new title compound 8-((4-((2,3-diaminopyridin-4-yl)-oxy)-3-fluorophenyl)-amino)-2-(4-fluorophenyl)-3-methyl-2,7-naphthyridin-1(2H)-one(C26H20F2N6O2, Mr = 486.48) has been prepared and determined by single-crystal X-ray diffraction. The crystal is of monoclinic, space group P21/c with a = 15.365(3), b = 13.144(2), c = 11.863(2), β= 108.882(3)°, Z = 4, V = 2267.0(7)3, Dc = 1.425 g/cm3, F(000) = 1008, μ = 0.105 mm-1, MoKa radiation(λ = 0.71073), R = 0.0480 and wR = 0.1294 for 3197 observed reflections with I 2σ(I). X-ray diffraction analysis reveals that the region C(substituents of 8-amino group and 3-methyl group on the 2,7-naphthyridin-1(2H)-one ring) of compound 6 are effectively planar. Intramolecular and intermolecular hydrogen bonds together with π···π interations are found in the structure. In addition, compound 6 shows potent c-Met and c-Kit kinase inhibition activities. 相似文献
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针对较大规模的成像系统提供了一种经济可行的集成方案。利用光纤的高速传输、USB的方便通用、SDRAM的大容量等特性,设计了前端控制板,完成了一个控制4 个CCD相机的功能,并在此基础上对原有软件进行修改,完成整体控制功能。通过增加前端控制板的设计,完成了基于光纤和USB的CCD集成系统,增加了系统的集成度,提高了系统的灵活性。In this paper an economical and feasible integration solution is introduced for large-scale CCD imaging system. Based on fiber transmission and USB technology, a front control point is designed which can control 4 CCD camera. The control software only has to do small modification for new change. With the design of front control point, a CCD integrated system is established with higher integration and more flexibility. 相似文献