CORRELATION OF ANNUAL CHANGE OF LUTEINIZING HORMONE-RELEASING HORMONE (LH-RH) WITH GONADAL DEVELOPMENT IN AMPHIOXUS

The present study demonstrates for the first time by RIA that LH-RH is present in the heads as well as the bodies of the amphioxuses of both sexes. Gonadoliberin increases gradually during the course of gonadal development. At the time of gonadal maturity, LH-RH content reaches a maximum. The annual change of LH-RH correlates well with gonadal development and the gonadosomatic index (GSI). The reproductive season of the amphioxus covers about three months from May to July, and LH-RH content starts to increase in May (66.89±5.26 ng) and reaches the peak in June (158.57±3.17 ng), indicating that LH-RH is likely also to be involved in the reproductive activity of the chordate. This finding is of significance in understanding the evolutionary process of the reproductive endocrine in the vertebrate.     

文昌鱼促黄体素释放素(LH-RH)的周年变化及其与性腺发育的相互关系

本文用放射免疫测定法首次发现,在性腺发育过程中,雌、雄文昌鱼头部的LH-RH含量和全身的LH-RH总含量逐渐增加。在性腺成熟时,LH-RH的含量达最大值。同时,文昌鱼LH-RH的周年变化与它的性腺发育周期和生殖指数十分一致。性腺发育周期的研究结果指出,文昌鱼的生殖季节为5月至7月,LH-RH含量在5月开始上升(66.89±5.26ng),6月达高峰(158.57±3.17ng)。这些结果指出文昌鱼的LH-RH在调控性腺发育和生殖活动中起重要作用。这对于了解文昌鱼在生殖内分泌进化中的地位及其与脊椎动物的亲缘关系方面也有重要意义。     

ULTRASTRUCTURAL STUDY OF THE HATSCHEK''''S PIT EPITHELIAL CELLS OF AMPHIOXUS

The authors have observed, using an electron microscope, that the number of thesecretory granules and the development of various organelles in the apical epithelial cellof the Hatschek's pit showed a positive correlation with the gonadal development andmaturity. At the same time, only the epithelial cells respond to GnRH-A(gonadotropin-releasing hormone analogue). Both cytological and endocrinological experiments demon-strate that the cell may be the primitive gonadotropic cell of amphioxus.     

文昌鱼卵母细胞发育成熟激素调控的研究

本文用电子显微镜技术和放射免疫测定法研究了文昌鱼卵母细胞发育成熟的激素调控。结果表明外源性GnRH-A可激发文昌鱼卵母细胞的发育成熟和产卵。用放射免疫测定法进一步揭示了GnRH-A可诱发性类固醇激素增加1—3倍,尤其17β-雌二醇的升高,与卵母细胞合成卵黄物质相一致。这些结果首次有力地证明文昌鱼卵母细胞发育成熟的激素调控与脊椎动物相类似,对文昌鱼生殖内分泌生理学有重要理论意义。     

文昌鱼哈氏窝上皮细胞超微结构的研究

本文用透射电子显微镜观察到文昌鱼哈氏窝顶部上皮细胞质中分泌颗粒生成的数量,各种细胞器的发育随性腺的发育和成熟,呈现一种正的相关性,同时,唯有这种细胞对GnRH-A发生应答。从细胞学和内分泌生理学方面证实,这种细胞可能是文昌鱼原始的促性腺激素分泌细胞。