肌苷和肌苷酸测定的新方法研究

采用紫外吸光光度法与高压纸电泳法联用，对次黄嘌呤类物质-肌苷及肌苷酸含量测定，试验了高压纸电泳法分离肌苷及肌苷酸的最佳条件，并对肌苷口服液等样品进行分离和测定。     

尿素与间甲酚共晶法分离间/对甲酚

Urea was dissolved into mixture of m/p-cresol(m/p = 59.1/40.9) upon heating to 80℃. The resulting solution was cooled spontaneously to 40℃, then further to a designated temperature in an even speed with stirring with even dropping of toluene. Co-crystal of urea and m-cresol was thus formed as white precipitate, which was then isolated by filution. Pure m-cresol was obtained by distillation of oil layer that was the upper layer of hydmlyzed decomposition of filtrating cake. Effects of solvent, material ratio, temperature on the yield and the purity were investigated, m-Cresol with purity of 99.0% and yield of 73.9 % is obtained at an optimized condition at urea/(m/p)-cresol = 23g/50ml,final temperature = - 15℃ ,time = 110min, and dropping of 150ml toluene.The existence of co-crystal of urea and m-cresol was verified by IR spectroscopy. The product purity was assayed by GC.