HORMONAL REGULATION OF CALLUS FORMATION IN RELATION TO NUCLEIC ACIDS AND AMINO ACIDS METABOLISM

The cotyledon segments of mung bean were cultured On Miller's medium with or without auxins and cytokinins. It was found that NAA and kinetiu were both required for callus formation and the growth rate increased proportionally to their concentrations. RNA and protein synthesis increased in the early stage of callus formation and the increase of the total RNA was mainly due to the increase of 25S and 18S RNA. In addition, it has been shown that the total amount of free amino acids in the control segments obviously increased after incubation and was larger than that in the callus-forming segments. On the other hand, the amount of protein amino acids in the callus-forming segments was larger than that in the control. Both proline and hydroxyproline existed in the control and callus-forming segments were increased, but the ratio of hydroxyprolinc and proline in the callus-forming segments was tfigher than that in the control.