CLONING IN Yersinia enterocolitica OF K99 ANTIGEN GENE FROM Escherichia coli

A recombinant plasmid pFS239 containing the geoe coding for K99 antigen of Escherichia coli and wide-host-range plasmid pKT230 has been cloned in E. coli C600. pFS239 has been transferred to Yersinia enterocolitica strains D29, L15 and L15 (pYV15) through triparental mating. In Y. enterocolitica transconjugants the expression of VW antigens and calcium dependence which represent the propertics associated with the virulence plastmid of Y. enterocolitica remains unchanged.     

大肠杆菌(Escherichia coli)K99抗原基因在小肠结肠炎耶氏菌(Yersinia enterocolitica)中的表达

本文通过SDS-聚丙烯酰胺凝胶电泳、双向免疫扩散及Western吸印实验,证明大肠杆菌(Escherichia coli)K99抗原基因在小肠结肠炎耶氏菌(Yersinia entero-colitica)无毒菌株D29(pFS239)和L15(pFS239)中表达,比较了K99基因在Y. enterocolitica与E. coli中表达条件的异同,并推测K99抗原蛋白在Y. ente-rocolitica中以纤毛的状态存在于细胞表面。本文是关于不同属的经克隆的基因在Y. enterocolitica中进行表达的首次报道。