Synthesis and Crystal Structure of [CpRu（η^5-C5H5）（dppc）Cl][PF6]

1INTRODUCTION The fragment[Ru(η5-C5H5)(PPh3)2Cl]plays an important role in the organo-ruthenium chemistry development,and has been used as a versatile star-ting material for other compounds due to its stability and facile manipulation.Much work has shown that a wide range of ligands with different steric and electronic properties can displace not only the Cl ligand but also the phosphine groups in[Ru(η5-C5H5)(PPh3)2Cl][1].The interest in this fragment and related derivatives concer…     

财经职校珠算教学的体会

贵阳市财经职业技术学校是在一九九0年创办的。学校为办出特色，十分重视珠算教学，明确提出了“打一手好算盘，写一手好字，做一篇好文章，记一本好帐”的要求，充分发挥珠算教师和班主任的作用，调动学生学习积极性，便珠算教学的质量有了很大的提高。     

对-硝基苯基荧光酮-溴化十六烷基三甲铵光度法测定锡的研究及其应用

本文研究了对-硝基苯基荧光酮在阳离子表面活性剂存在下与锡的胶束增敏显色反应。结果表明,对-硝基苯基荧光酮与溴化十六烷基三甲铵在0.4—0.75mol/L硫酸介质中,可以与锡形成三元络合物,其最大吸收峰在514—516nm,络合物的表观摩尔吸光系数为9.1×10~4。方法的选择性良好。锑(Ⅲ)干扰严重,可用高锰酸钾氧化消除,利用上述显色反应,拟定了黄铜和纯铜中锡的分光光度测定法,并取得了令人满意的结果。     

锑(Ⅲ)-邻-硝基苯基荧光酮-乳化剂OP光度法的研究及其应用

本文研究了在乳化剂OP存在下,Sb与邻-硝基苯基荧光酮(O-NPF)的显色反应。结果表明,在0.14—0.28mol/L硫酸介质中,Sb(Ⅲ)与O-NPF及乳化剂OP形成三元络合物,最大吸收峰为515nm,(?)_(515)=6.67×10~4,Sb浓度在0—5微克/25毫升范围內服从比尔定律芯苛烁扇爬胱蛹跋扇诺姆椒?拟定了不含锡的纯铜及黄铜中微量锑的测定方法。     

一种1,8-萘酰亚胺衍生物检测半胱氨酸荧光探针 的合成及其在活细胞和秀丽隐杆线虫中成像中的运用

基于1,8-萘酰亚胺衍生物,构建了一种检测半胱氨酸(Cys)的新型荧光探针TPFC-Acryloyl。光谱研究表明该探针能有效识别Cys且能够在1min内实现快速响应。探针对Cys的检测表现出高选择性,检测限为2.13μmol/L。经荧光光谱和质谱实验确证其检测机理为：Cys与TPFC-Acryloyl分子中的丙烯酸酯发生共轭加成-环化反应,进而羟基裸露的同时释放出黄色荧光。细胞毒性测试表明探针TPFC-Acryloyl的细胞毒性低。此外,该探针还被成功应用于活细胞和秀丽隐杆线虫中Cys的荧光成像。     

Synthesis of a fluorescent probe based on naphthalimide derivative for simultaneous detecting of NO and pH in bioimagingEI北大核心CSCD

A new type of fluorescent probe Na-pHNO was synthesized for the simultaneous detection of NO and pH based on naphthalimide derivative. The probe had good fluorescence property in mixture solution of 99% phosphate buffer solution（PBS）and 1% dimethyl sulfoxide（DMSO）solution. It can produce a yellow-green emission peak at 544 nm,and the fluorescence intensity can be enhanced with the increase of NO concentration or the decrease of pH. The probe Na-pHNO displayed good selectivity,high sensitivity,and strong anti-interference towards NO,exhibiting good fluorescence reversibility at pH 5.5-8.5. The spectral studies showed that the probe Na-pHNO exhibited weak fluorescence due to a dual photoinduced electron transfer（PET）effect. After interacting with NO or pH,the dual PET inhibitory effect occurred,and the fluorescence intensity was significantly enhanced. The fluorescence imaging of probe Na-pHNO in HeLa cells showed that it could realize the visual monitoring of the changes of intracellular pH and NO concentration. The probe has been successfully applied to fluorescence imaging of Caenorhabditis elegans. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

基于苯并吡喃腈-香豆素体系的近红外比率型荧光探针用于检测He La细胞中的过氧亚硝酸盐(英文)

过氧亚硝酸根(ONOO-)是生物体内的一种重要的活性氧,与人体内的各种生理活动息息相关.利用苯并吡喃腈-香豆素体系,合成了一种用于ONOO-检测的近红外比率型荧光探针(E)-7-(二乙胺基)-3-(2-(苯并吡喃腈)乙烯基)-香豆素(DCCM).该探针在ONOO-存在下表现出强烈的响应,颜色从紫色变为浅粉色,最大发射峰蓝移217nm,荧光颜色从淡紫色变为蓝色,能够直观地对溶液中的ONOO-进行监测. DCCM可以灵敏地检测ONOO^-,最低检测限为6.0×10^-7 mol·L^-1,该探针被成功用于HeLa细胞中的ONOO-成像检测.     

连续扫描ICP-AES法测定铜合金中铝铁钴镍和硅

采用快速自动扫描等离子体光谱仪测定新型铜合金中铝、铁、钴、镍和硅,分析速度快,准确度较好。一、仪器及工作条件 PerKin-Elmer ICP-6500型光谱仪,Perkin-Elmer7300计算机,PR-210打印机,炬管组合配有氧化铝注入管,内径2.00mm。工作条件见表1。