液相色谱中保留值变化规律——某些PTH-氨基酸最佳冲洗剂配比的选择

本文根据基本方程Ink′=b_0 b_1cB b_2IncB以及色谱柱的总分高效能指标K_1,计算出多元组份分离时所需的最小相对保留值a_(min)和最难分离物质对的相对保留值a′.由a′必须大于′a_(min)以及最后流出组份k′_(lasl)最小的最佳冲洗剂组成选择原则,建立起以最少的实验数据快速进行最佳冲洗剂组成选择的方法.文中给出了以甲醇、乙醚、已烷作三元冲洗剂,以5对氨基酸作样品,进行最佳冲洗剂组成选择的例子,色谱图表明:理论预测与实验结果吻合。     

求液固色谱保留值方程的简便方法

硅胶柱是液固色谱的主要柱型。改变流动相以改变选择性,是液相色谱的特色。为了选择最佳操作条件,R.L.Snyder首先提出适用于弱极性及中等强度极性流动相的保留值方程,后来他用综合的模式解释保留值与流动相组分的关系。Glajch等采用统计学方法设计混合溶剂,通过7次溶剂试     

智能色谱法的发展（上）

本文综述了我们在智能色谱方面的发展,包括硬件和软件两个主要部分.第一部分评述了细管径高效液相色谱柱,不可抽提的气相色谱毛细管柱,无阀切换技术,四合一组合检测器和快速扫描紫外光谱检测器;第二部分包括三个应用软件:保留值预测.最佳操作条件预测和全谱图拟含软件,并用大量实验证明了保留值方程的正确性。这些方程用于定性分析及最佳条件选择,文中举出了有关的例子,讨论了细管径高效液相色谱,不可抽提的气相毛细管柱、柱切换系统、组合检测器、快速扫描紫外检测器、曲线拟合方法在智能色谱中的应用。     

描述多元流动相组成与液-固色谱保留值规律的基本方程

本文利用弱电解质的离子平衡原理与化学动力学的方法,处理了包括离子平衡在内的液-固色谱保留值的规律,得到了弱电解质溶液在内的多元流动相成和色谱保留值规律的关系式,所得实验数据且给以良好地佐证。     

RETENTION RULE IN THE LIQUID CHROMATOGRAPHY——SELECTION OF THE OPTIMUM ELUTION COMPOSITION OF SOME PTH AMINO ACIDS ON SILICA GEL

The minimal relative retention α' and the required relative retention α'_(min) of the pair of substances being most difficult to separate in a multicomponent mixture have been discussed on the basis of the fundametal retention equation ln k'=b_0 b_1C_B b_2ln C_B and resolution criterion. The selection principle to optimize elution composition means that selected a value must be greater than α'_(min) and that the capacity factor of last peak should be held to a minimum. A rapid method to select optimum elution composition has been established in which it is necessary to do a few experiments. An example for the 5 pairs of phenylthiohydantion amino acids (PTH-AA) using the ternary mobile phase of methanol, ether and hexane is also demonstrated in this paper. The results show that theoretical prediction is good in agreement with the experiments.     

智能色谱法的发展（下）

二、智能色谱软件的发展 发展智能色谱,硬件固然十分重要,它为先进的软件提供了技术保证,但发展的关键又在于软件。我们的色谱软件系统的发展基础是色谱多元组分分离理论。     

A FUNDAMENTAL EQUATION FOR DESCRIBING THE RELATIONSHIP BETWEEN THE COMPOSITION OF THE MULTICOMPONENT MOBILE PHASE AND CHROMATOGRAPHIC RETENTION

A fundamental equation for discribing the relationship between the composition of the multicomponent mobile phase (CMCMP) and chromatographic retention (CRV)is derived by using statistical thermodynamic method. The equation is proven by a ternary mobile phase experiment for 11 compounds. Based on this equation, we also obtain retention equations for weak dissociative solute.