LIQUID AND SOLID TWO-PHASE RADIOIMMUNOASSAY——SIMULTANEOUS MEASUREMENT OF LEVELS OF TRIIODOTHYRONINE AND THYROXINE OF HUMAN SERUM IN A SINGLE SPECIMEN

The principle of the liquid and solid two-phase radioimmunoassay and its application to measuringthe concentrations of triiodothyronine and thyroxine of human serum in a single sample at the sametime are described in this paper. Rabbit anti-triiodothyronine antiserum was immobilized on the innersurface of a plastic tube as solid-phase radioimmunoassay system of testing triiodothyronine and donkeyanti-rabbit gamma globulin antiserum was used to separate immunocomplex in liquid-phase radioim-munoassay system of thyroxine reaction. In both systems, radioiodine-125-labelled antigens were usedas tracer. Through simple calculation, we can ger the results of serum concentrations of triiodothyro-nine and thyroxine in a single sample. Compared with classical single-component radioimmunoassay oftriiodothyronine and thyroxine and by methodological appraisal of two-phase radioimmunoassay itself,this method has the advantages of very high efficiency, reliability and also has the same specificityand precision.     

液固两相双组份放射免疫分析法——同时测定甲状腺素和三碘甲腺原氨酸的研究

本文叙述了液固两相双组份放射免疫分析技术的基本原理,报道了应用此法同时测定单位血清样本中人血清甲状腺素(T4)和三碘甲腺原氨酸(T3)浓度的结果。将兔抗T3血清涂布至塑料试管内壁,组成测定T3的固相放射免疫系统;用驴抗兔第二抗体作为测定T4液相放射免疫系统中的分离试剂。两个测定体系均使用放射性~(125)I标记抗原。经简单计算,可由单位样本得血清T3和T4两者的浓度。所得结果经与经典的单组份放射免疫分析法测定值比较,以及本身的方法学考核证明,液固两相双组份放射免疫分析技术除具有与经典方法同样的特异性和精确性外,还具有用血清量少、效率高和简便易行等优点。